The Isoflavones Genistein and Daidzein Increase Hepatic Concentration of Thyroid Hormones and Affect Cholesterol Metabolism in Middle-Aged Male Rats.
2019
Аутори:
Šošić-Jurjević, BrankaLütjohann, Dieter
Renko, Kostja
Filipović, Branko
Radulović, Niko
Ajdžanović, Vladimir
Trifunović, Svetlana
Nestorović, Nataša
Živanović, Jasmina
Manojlović-Stojanoski, Milica
Kӧhrle, Josef
Milošević, Verica
Тип документа:
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт:
We examined whether isoflavones interfere with thyroid homeostasis, increase hepatic thyroid hormone concentrations and affect cholesterol metabolism in middle-aged (MA) male rats. Thirteen-month-old Wistar rats were injected subcutaneously with 35 mg/kg b.w./day of genistein, daidzein or vehicle (controls) for four weeks. Hepatic Dio1 gene expression was up-regulated by 70% (p < 0.001 for both) and Dio1 enzyme activity increased by 64% after genistein (p < 0.001) and 73% after daidzein treatment (p < 0.0001). Hepatic T3 was 75% higher (p < 0.05 for both), while T4 increased only after genistein treatment. Serum T4 concentrations were 31% lower in genistein- and 49% lower in dadzein-treated rats (p < 0.001 for both) compared with controls. Hepatic Cyp7a1 gene expression was up-regulated by 40% after genistein and 32% after daidzein treatment (p < 0.05 for both), in agreement with a 7α-hydroxycholesterol increase of 50% (p < 0.01) and 88% (p < 0.001), respectively. Serum 24- and 27-hydroxycholesterol were 30% lower (p < 0.05 for both), while only 24-hydroxycholesterol was decreased in the liver by 45% after genistein (p < 0.05) and 39% (p < 0.01) after dadzein treatment. Serum concentration of the cholesterol precursor desmosterol was 32% (p < 0.05) lower only after dadzein treatment alone, while both isoflavones elevated this parameter in the liver by 45% (p < 0.01). In conclusion, isoflavones increased T3 availability in the liver of MA males, despite decreasing serum T4. Hepatic increase of T3 possibly contributes to activation of the neutral pathway of cholesterol degradation into bile acids in the liver. While isoflavones obviously have the potential to trigger multiple mechanisms involved in cholesterol metabolism and oxysterol production, they failed to induce any hypocholesterolemic effect.
Кључне речи:
Cholesterol metabolism; Daidzein; Genistein; Liver; Middle-aged rats; Thyroid homeostasisИзвор:
The Journal of Steroid Biochemistry and Molecular Biology, 2019, 190, 1-10Финансирање / пројекти:
- Одговор неуроендокриног система пацова на одабране биљне екстракте, фитоестрогене, стероидне и пептидне хормоне (RS-MESTD-Basic Research (BR or ON)-173009)
- European Society for Paediatric Endocrinology
- Sociedade Portuguesa de Pediatria
- Deutsche Forschungsgemeinschaft (TP3,DFG-GK 1208,RE3038/1-1)
Повезане информације:
- Друга верзија
https://radar.ibiss.bg.ac.rs/handle/123456789/3305
DOI: 10.1016/j.jsbmb.2019.03.009
ISSN: 0960-0760
PubMed: 30885834
WoS: 000471087300001
Scopus: 2-s2.0-85063079407
URI
internal-pdf://Šošić-Jurjević et al. - 2019 - The Isoflavones Genistein and Daidzein Increase Hepatic Concentration of Thyroid Hormones and Affect Chol.pdfhttps://www.sciencedirect.com/science/article/pii/S0960076018307507
https://radar.ibiss.bg.ac.rs/handle/123456789/3290