l-Arginine Induces White Adipose Tissue Browning-A New Pharmaceutical Alternative to Cold.
2022
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
The beneficial effects of l-arginine supplementation in obesity and type II diabetes involve white adipose tissue (WAT) reduction and increased substrate oxidation. We aimed to test the potential of l-arginine to induce WAT browning. Therefore, the molecular basis of browning was investigated in retroperitoneal WAT (rpWAT) of rats exposed to cold or treated with 2.25% l-arginine for 1, 3, and 7 days. Compared to untreated control, levels of inducible nitric oxide (NO) synthase protein expression and NO signaling increased in both cold-exposed and l-arginine-treated groups. These increases coincided with the appearance of multilocular adipocytes and increased expression levels of uncoupling protein 1 (UCP1), thermogenic and beige adipocyte-specific genes (Cidea, Cd137, and Tmem26), mitochondriogenesis markers (peroxisome proliferator-activated receptor (PPAR)-γ coactivator-1α, mitochondrial DNA copy number), nuclear respiratory factor 1, PPARα and their respective downstream lipid oxidation enzymes after l-arginine treatment. Such browning phenotype in the l-arginine-treated group was concordant with end-course decreases in leptinaemia, rpWAT mass, and body weight. In conclusion, l-arginine mimics cold-mediated increases in NO signaling in rpWAT and induces molecular and structural fingerprints of rpWAT browning. The results endorse l-arginine as a pharmaceutical alternative to cold exposure, which could be of great interest in obesity and associated metabolic diseases.
Кључне речи:
Browning; l-arginine; Nitric oxide; ObesityИзвор:
Pharmaceutics, 2022, 14, 7, 1368-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- WARMED - Development of No-Based Approaches for Guided White Adipose Tissue Browning. Can We Tackle Metabolic Diseases By Heating Up/Cooling Down the Fat? (RS-ScienceFundRS-Promis-6066747)
DOI: 10.3390/pharmaceutics14071368
ISSN: 1999-4923
PubMed: 35890263
WoS: 000833724100001
Scopus: 2-s2.0-85133487097
URI
https://www.mdpi.com/1999-4923/14/7/1368http://www.ncbi.nlm.nih.gov/pubmed/35890263
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9324995
http://radar.ibiss.bg.ac.rs/handle/123456789/5071