Detection of DNA methylation profiles in mouse embryonic fibroblasts using Fourier transform-infrared spectroscopy
Аутори:
Sarić, AnaTolić, Anja
Grdović, Nevena
Dinić, Svetlana
Rajić, Jovana
Đorđević, Miloš
Đorđević, Marija
Arambašić Jovanović, Jelena
Mihailović, Mirjana
Poznanović, Goran
Uskoković, Aleksandra
Vidaković, Melita
Dučić, Tanja
Остала ауторства
Vasiljević, BrankaPatenković, Aleksandra
Nikolić, Nađa
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2019 by the Serbian Genetic Society
Метаподаци
Приказ свих података о документуАпстракт:
Epigenetic processes orchestrate the cell type-specific use of the genetic information
essential for normal development and for maintaining the overall integrity of the genome.
DNA methylation is probably the most extensively studied epigenetic mark and represents
the covalent attachment of a methyl group to cytosine that is located next to guanine within
the genomic DNA. The alteration of DNA methylation patterns by hyperglycaemia, oxidative
stress and inflammation may have potential epigenetic impacts on gene regulation in diabetic
individuals. Further research devoted to improve the steps that could be undertaken in the
early diagnosis, prevention and treatment of diabetes and its complications is a scientifically
and socially significant task. We used the Fourier transform-infrared (FTIR) spectroscopy
(ALBA synchrotron, Cerdanyola del valles, Spain) for qualitative spectral analysis of
normomethylated DNA from mouse fibroblast cells (NIH3T3) and hypomethylated DNA
from PARP-1 knockout mouse fibroblast cells (PARP-/-). We obtained the global information
regarding the DNA methylation profiles in mouse fibroblast cells by FTIR spectroscopy that
was visualised by immune-fluorescent staining using anti-methyl cytosine (5mC) antibody.
Some differences in DNA methylation profiles between examined cell lines were observed
in spectral region significant for cytosine (990-1250 nm). The most interesting picks were
observed approximately at wavelength: 1240 nm, 1150 nm, 1110 nm and 1010 nm. These
results are in the same time a verification of the proof of principle for FTIR based analysis of
the differences between normomethylated and hypomethylated genomic DNA which could
be set as a potential predictive and diagnostic tool in future. To our knowledge this is a first
time that synchrotron-based FTIR micro-spectroscopy is used for detection of the presence
of 5mC and changes in the DNA methylation profile in cells.
Кључне речи:
epigenetics; DNA methylation; FT-IR spectroscopyФинансирање / пројекти:
- Detection of DNA methylation profile changes using FTIR micro-spectroscopy: a method for possible implementation as a diagnostic tool in diabetes, ALBA Synchrotron, BL01 – MIRAS, Project No.: 2018093034
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200110 (Универзитет у Београду, Медицински факултет) (RS-MESTD-inst-2020-200110)
У:
- Vasiljević B, Patenković A, Nikolić N, editors. 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia. Belgrade: Serbian Genetic Society; 2019. p. 75.