Microglial morphological response to the lack of direct social contact in periadolescent rats
2023
Аутори:
Potrebić, MilicaPavković, Željko
Srbovan, Maja
Dubljević, Olga
Pešić, Vesna
Остала ауторства
Kanazir, SelmaSavić, Danijela
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2023 by Serbian Neuroscience Society and associates
Метаподаци
Приказ свих података о документуАпстракт:
Aim: Microglia are macrophages of the brain with a fundamental role in neuronal
homeostasis. Stress-induced activation of microglia leads to rapid morphological
changes and it is implicated that these alternations could mediate different aspects of
early-life stress psychopathologies. We aimed to examine morphological response of
hippocampal microglia to social isolation using peripubertal rats as a rodent model of
adolescence.
Methods: Male peripubertal Wistar rats were randomly selected for group-housing
(GH; n=3 per cage) or single-housing (SH; n=1 per cage) at postnatal day 29. After
1st and 2nd week of defined housing animals῾ brains were sampled, and used for
microglia iba-1 fluorescent labelling and morphology analysis: one filed per section
per animal (n=4 animals per group) in hippocampal dentate gyrus (HDG). Data for
microglia cell shape (FracLac plugin, ImageJ) and cell ramification (Analyze Skeleton
plugin, ImageJ) were collected from single cells in photomicrograph visualized with a
confocal microscope (Leica Microsystems).
Results: Compared to GH peers, SH rats had lower microglial cell density in the HDG
after the 1st week of defined housing, which was not evident after the 2nd week. Other
parameters of microglia shape and ramification showed no difference between the
groups in neither of the two time points examined.
Conclusions: Obtained results showed that change in the cell density in the HDG was
the only and transient change in microglia morphology due to the social isolation
during adolescence. Further examination of other stress-related brain regions is
needed to define in depth the relationship between the microglia phenotype and earlylife
stress conditions.
Кључне речи:
Microglia; Adolescence; Earlylife stressФинансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200017 (Универзитет у Београду, Институт за нуклеарне науке Винча, Београд-Винча) (RS-MESTD-inst-2020-200017)
У:
- Kanazir S, Savić D, editors. Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. Belgrade: Serbian Neuroscience Society; 2023. p. 108.