Protein tyrosine phosphatase receptors N and N2 regulate gonadotropin-releasing hormone neuron function
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2023
Аутори:
Sokanović, Srđan![](/themes/MirageIBISS/images/orcid.png)
Constantin, Stephanie
Lamarca Dams, Aloa
Mochimaru, Yuta
Smiljanić, Kosara
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Bjelobaba, Ivana
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Prévide, Rafael
Stojilković, Stanko
Остала ауторства
Kanazir, SelmaSavić, Danijela
Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2023 by Serbian Neuroscience Society and associates
Метаподаци
Приказ свих података о документуАпстракт:
Simultaneous knockout of the neuroendocrine marker genes Ptprn and Ptprn2, which
encode the protein tyrosine phosphatase receptors N and N2, respectively, causes
infertility of female mice while males are fertile. To clarify the mechanism of sex specific roles of Ptprn and Ptprn2 in mice reproduction, we analyzed the effects of
their double knockout (DKO) on the hypothalamic-pituitary-gonadal axis. In DKO
females, a delay in puberty and lack of ovulation were observed, supplemented by
changes in ovarian gene expression and steroidogenesis. In DKO males, the testicular
gene expression, steroidogenesis, and development of reproductive organs were not
affected. However, in both sexes, pituitary luteinizing hormone (LH) beta gene
expression and LH levels were reduced, while the calcium-mobilizing and LH
secretory actions of gonadotropin-releasing hormone (GnRH) receptors were
preserved. The expression of hypothalamic Gnrh1 and Kiss1 genes were also reduced
in DKO females and males. The density of immunoreactive GnRH fibers was
decreased in the median eminence in DKO females and males. The density of
immunoreactive kisspeptin fibers was also decreased in the rostral periventricular
region of the third ventricle of females and in the arcuate nucleus of females and
males. Therefore, infertility in DKO females cannot be explained only by sex-specific
gonadotroph impairment. Instead, changes in hypothalamic gene expression,
specifically Kiss1 in the rostral periventricular region of the third ventricle, might
provide an alternative hypothesis due to its sexual dimorphism and involvement in
puberty onset and ovulation.
Кључне речи:
Pituitary luteinizing hormone; Gonadotropin-releasing hormone; Protein tyrosine phosphatase receptors; Kiss1У:
- Kanazir S, Savić D, editors. Book of abstracts: 8th Congress of Serbian Neuroscience Society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. Belgrade: Serbian Neuroscience Society; 2023. p. 107.