Faecal pollution as potential driver of antibiotic resistance genes in the Danube River
2022
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Аутори:
Schachner, IrisKolm, Claudia
Vierheilig, Julia
Savio, Domenico
Zarfel, Gernot
Koller, Michael
Kittinger, Clemens
Jakwerth, Stefan
Linke, Rita
Kolarević, Stoimir
Kračun-Kolarević, Margareta
Tóth, Erika
Farnleitner, Andreas H.
Kirschner, Alexander K.T.
Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2022 by the Serbian Society of Microbiology
Метаподаци
Приказ свих података о документуАпстракт:
Human-induced antimicrobial resistance is an emerging concern in aquatic environments. Faecal pollution
sources represent potential propagation pathways, however large-scale quantitative studies in whole river
systems are missing. In the course of the Joint Danube Survey, the Danube River was investigated for the
fourth time in summer 2019 from its headwaters to the delta. Microbial-faecal pollution patterns and the
occurrence of selected antibiotic resistance genes (ARGs) were studied. Subsequently, a one-year time series analysis at selected relevant sites allowed to assess temporal variability.
The intestinal bacterium Escherichia coli was quantified according to standard cultivation methods as indicator for total faecal pollution. In general, longitudinal patterns of faecal pollution showed a comparable picture to previous Joint Danube Surveys, with low to moderate pollution in the upper reaches (Germany, Austria) and critical to strong pollution in the middle and lower sections, especially in Serbia. Genetic microbial source tracking methods, determined via quantitative PCR, allowed us to define human faecal pollution as dominant pollution source along the whole Danube. This trend could be generally confirmed during the annual sampling campaign. To estimate the potential of permanent colonialization of faecal microorganisms within the Danube River ecosystem, biofilms as potential reservoirs were studied along the course of the entire river. Here, E.coli were ubiquitously present but highly variable. Ongoing analyses on the occurrence and quantity of selected ARGs will identify hotspots of antimicrobial resistance and their relationship to faecal pollution along the Danube River and thereby contribute to guide future monitoring and management strategies.
Финансирање / пројекти:
- FWF-Project P32464
У:
- Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia. Belgrade: Serbian Society of Microbiology; 2022. p. 137-8.