Comparative in vitro evaluation of genotoxic potential of the Danube River water extracts in prokaryotic and eukaryotic assays
2022
Authors:
Kolarević, StoimirKračun-Kolarević, Margareta
Oswald, Peter
Slobodnik, Jaroslav
Paunović, Momir
Vuković-Gačić, Branka
Žegura, Bojana
Document Type:
Conference object (Published version)
,
© 2022 by the Serbian Society of Microbiology
Metadata
Show full item recordAbstract:
BACKGROUND
Prokaryotic assays are of great interest in eco/geno-toxicology as they are short-term, simple and cost
effective. Application of strains of Salmonella typhimurium with modifications such as increased cell wall
permeability and lack of excision repair system is common for the detection of genotoxicity. Various eukaryotic models have been developed lately, such as fish derived cell lines, which can mimic the response of the aquatic organisms. Considering different level of cell organization among these models, difference in sensitivity is expected, yet only few studies address this topic.
OBJECTIVES
The major objective was to investigate genotoxic potential of extracts of surface water of the Danube
and to compare the results obtained in applied prokaryotic and eukaryotic models.
METHODS
Surface water samples from 24 sites along the Danube River were extracted by LVSPE Horizon sampler providing relative enrichment factor (REF) of the extracts of 25,000x. For representative prokaryotic model the SOS/umuC assay with S. typhimurium TA1535/pSK1002 was used. Eukaryotic model comprised assessment of cytotoxicity by MTS test and assessment of genotoxicity by comet assay in ZFL (ZebraFish Liver) cells.
RESULTS
While none of the tested samples has induced SOS response, the result of MTS assay indicated that 11 of 24 samples were cytotoxic. In comet assay, genotoxic potential was found in 9 of 24 samples. Results indicated higher sensitivity of used eukaryotic model in comparison with prokaryotic. Applied prokaryotic assay is often suggested to be used as prescreening tool in diagnostics of genotoxic potential which by our opinion could be misleading.
Keywords:
genotoxicity; Danube River; SOS/umuC; zebrafishFunding / projects:
- This research was supported by FEMS Research Training Grant program.
In:
- Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia. Belgrade: Serbian Society of Microbiology; 2022. p. 160-1.