Occurrence of antibiotic resistance genes along gradients of faecal pollution in water and biofilm samples from the whole Danube River
2023
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Аутори:
Schachner-Gröhs, IrisKolm, Claudia
Vierheilig, Julia
Leopold, Melanie
Zarfel, Gernot
Koller, Michael
Kittinger, Clemens
Jakwerth, Stefan
Linke, Rita
Kolarević, Stoimir
Kračun-Kolarević, Margareta
Toth, Erika
Farnleitner, Andreas
Kirschner, Alexander
Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2023 by the Austrian Committee - International Association for Danube Research
Метаподаци
Приказ свих података о документуАпстракт:
Antimicrobial resistance (AMR) represents one of the top ten global public health threats according
to the WHO. The spread of resistances is no longer limited to clinical settings and the natural
environment, especially aquatic ecosystems, are also strongly involved in the global spread. Holistic
as well as quantitative studies are still scarce. We here present for the first time a large scale study
on the Danube River regarding the distribution of AMR along different faecal pollution patterns.
For this study, samples from an international sampling campaign (Joint Danube Survey 4) as well as
additional seasonal samples were screened for the occurrence and origin of faecal pollution and for
the concentrations of nine representative antibiotic resistance genes (ARGs). In addition to water
samples also river biofilms were monitored to account for both, temporal and permanent river
habitats. Analyses were amended by a set of different cultivation-based and non-cultivation-based
microbiological and environmental data to explain the observed pattern in faecal pollution and ARG
concentrations.
Faecal pollution exists and varied strongly along the whole Danube river; hotspots could be identified
downstream of metropolitan areas at which also substantial concentrations of ARGs could be
detected. Quantifying concentrations of ARGs conferring resistance to different antibiotic classes
revealed strong differences between the different genes. Comparing their presence in water and
biofilm samples, allowed to assess how stably present these genes already are in the Danube River
ecosystem.
Environmental surveys are necessary to establish environmental baselines, to be able to guide future
AMR monitoring as well as to enforce useful management strategies. This integrative study therefore
provides first insights in the antimicrobial resistance situation in the most international river in the
world and second largest river in Europe
У:
- Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. Austrian Committee - International Association for Danube Research; 2023. p. 25.