Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats
2024
Аутори:
Šošić-Jurjević, BrankaBorković-Mitić, Slavica
Pavlović, Slađan
Vlahović, Dragana
Miler, Marko
Cesar, Thais
Ajdžanović, Vladimir
Milenković, Dragan
Stellaard, Frans
Trifunović, Svetlana
Filipović, Branko
Dieter, Lutjohann
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
This study aimed to assess the antioxidant capacity of lemon flavonoid extract Eriomin®
(LE) and its impact on cholesterol metabolism in the context of healthy aging. We orally treated 24-
month-old male Wistar rats with an LE (40 mg/kg) suspended in 0.3 mL of sunflower oil. At the
same time, control groups received an equal volume of sunflower oil (CON) or remained untreated
(ICON) daily for 4 weeks. We examined LE’s effects on superoxide dismutase and catalase- and
glutathione-related enzyme activities, the concentration of lipid peroxides and protein carbonyls,
total oxidant status (TOS) and antioxidant status (TAS), and oxidative stress index (OSI) in the liver,
jejunum, and ileum. We also measured total cholesterol, its biosynthetic precursors (lanosterol,
lathosterol, desmosterol), its degradation products (bile acid precursors) in the serum, liver, jejunum, and ileum, and serum phytosterols (intestinal absorption markers). LE reduced TOS, TAS, and
OSI (p < 0.05) compared with control values, indicating its consistent antioxidant action in all examined organs. LE lowered hepatic desmosterol (p < 0.05) while also reducing 7α- and 24-hydroxycholesterol levels in the liver and ileum (p < 0.01). Serum cholesterol, hepatic gene expression, and the
immunostaining intensity of CYP7A1 were unchanged. In conclusion, LE exerted non-enzymatic
antioxidant effects and reduced cholesterol degradation, reducing its biosynthesis products, thereby
maintaining serum cholesterol levels.
Кључне речи:
lemon flavonoids; Eriomin; aging; rat; liver; oxidative stress; cholesterol; desmosterol; 7α-hydroxycholesterol; CYP7A1Извор:
International Journal of Molecular Sciences, 2024, 25, 10, 5221-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)