Immunohistomorphometric Study of GH Cells after Treatment with Genistein in an Andropause Animal Model
2012
Преузимање 🢃
Аутори:
Milošević, VericaTrifunović, Svetlana
Bijelić, Dunja
Popovska-Perčinić, Florina
Nestorović, Nataša
Sekulić, Milka
Ajdžanović, Vladimir
Тип документа:
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Introduction: Andropause, the culminating phase of ageing in males, implies the decline of growth hormone (GH)/insulin-like growth factor-1 (IGF-1) axis, which is responsible for somatic development. On the other hand, the soybean phytoestrogen genistein is increasingly consumed as an alternative therapeutic for age-related diseases, namely cardiovascular conditions, cancer, and osteoporosis. The aim of our study was to examine the effects of genistein on the immunohistomorphometric features of GH cells in an andropause animal model.
Methods and Materials 16-month-old Wistar rats were divided into sham-operated (SO), orchidectomized (Orx), and genistein-treated orchidectomized (Orx+G) groups. Genistein (30 mg/kg/day) was administered subcutaneously for 3 weeks, while the SO and Orx groups received the vehicle alone. GH cells were identified by the peroxidase-antiperoxidase (PAP) immunohistochemical procedure.
Results In the Orx+G group, GH cell volumes as well as the relative volume densities were significantly (p<0.05) decreased by 18% and by 62%, respectively, in comparison with the same parameters of SO animals. Compared to Orx animals, the cell volumes and relative volume densities of GH cells in the Orx+G group were also significantly (p<0.05) decreased by 14% and by 65%, respectively.
Conclusion It can be concluded that chronically applied genistein suppresses the immunohistomorphometric parameters of pituitary GH cells in an andropause animal model.
Финансирање / пројекти:
- Одговор неуроендокриног система пацова на одабране биљне екстракте, фитоестрогене, стероидне и пептидне хормоне (RS-MESTD-Basic Research (BR or ON)-173009)
У:
- Abstracts: 15th Congress of the European NeuroEndocrine Association: ENEA 2012; 2012 Sep 12-15; Vienna, Austria. Gablitz: Krause & Pachernegg GmbH; 2012. p. 87. (Journal of Clinical Endocrinology and Metabolism; Vol. 5; Spec.Issue 3).