Vitamin D3 application and factors of its activity in the adrenal cortex of andropausal rats: A functionally-histological study
2024
Аутори:
Ajdžanović, VladimirŠošić-Jurjević, Branka
Živanović, Jasmina
Miler, Marko
Stanković, Sanja
Ranin, Jovan
Filipović, Branko
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2024 Elsevier GmbH.
Метаподаци
Приказ свих података о документуАпстракт:
Introduction and aim.Vitamin D supplementation in aging subjects manifests the positive effect on various health-related parameters. We performed functionally-histological analysis of the adrenal cortex regarding the factors of vitamin D activity and corticosterone output, after vitamin D3 application in a rat model of the andropause.Material and methods.Middle-aged Wistar rats were divided into sham operated (SO; n=8), orchidectomized (Orx; n=8) and vitamin D3 treated orchidectomized (Orx+vit. D; n=8) groups. Vitamin D3 (5 μg/kg b.m.) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using histochemistry/immunohistochemistry, stereology, ultrastructural and biochemical analyses.Results.Orchidectomy (Orx) decreased the adrenal cortex-related volume densities of vascular (p<0,0001), vitamin D receptor (VDR; p<0,0166), cytochrome P450 oxidase 2R1 (CYP 2R1; p<0,0001) and cytochrome P450 oxidase 24 (CYP 24; p<0,0001) depots, but increased the volume density of cytochrome P450 27B1 (CYP 27B1; p<0,0001) depots. In Orx+vit. D rats, increase of the adrenal cortex-related volume densities of collagen (p<0,0001), VDR (p<0,0001) and CYP 2R1 (p<0,0001) depots as well as the lipid-droplet diameter (p<0,0001) in adrenocortical outer zona fasciculata cells was observed, while a decrease of volume densities of the vascular (p<0,0001), CYP 27B1 (p<0,0001) and CYP 24 (p<0,0001) depots was registered, all versus Orx group. Plasma level of ACTH was decreased (p=0,0155) and serum concentrations of 25-hydroxyvitamin D3 and corticosterone were increased (p<0,0001 and p=0,0187, respectively), all after the same treatment.Conclusions.Increased corticosterone output after vitamin D3 application to andropausal rats appears not to be related to increased availability of 25-hydroxyvitamin D3 and decreased degradation of 1,25-dihydroxyvitamin D3 in adrenal tissue, but rather involves the central regulatory mechanisms.
Кључне речи:
Adrenal cortex; Vitamin D3; Enzymes; Rats; AndropauseИзвор:
Annals of Anatomy, 2024, 256, 2024, 152322-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
Повезане информације:
- Друга верзија
https://radar.ibiss.bg.ac.rs/handle/123456789/6999
DOI: 10.1016/j.aanat.2024.152322
ISSN: 0940-9602
PubMed: 39182585