Prikaz osnovnih podataka o dokumentu
The horse chestnut lines harboring the rol genes
dc.creator | Zdravković-Korać, Snežana | |
dc.creator | Ćalić, Dušica | |
dc.creator | Druart, PH | |
dc.creator | Radojević, Ljiljana R. | |
dc.date.accessioned | 2017-11-23T11:15:28Z | |
dc.date.available | 2015-11-17T10:26:51Z | |
dc.date.issued | 2003 | sr |
dc.identifier.issn | 0006-3134 | sr |
dc.identifier.other | Rad_konverzija_3772 | sr |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/1777 | |
dc.description.abstract | An Agrobacterium rhizogenes-mediated transformation system for Aesculus hippocastanum L. has been developed. Wounded androgenic embryos of A. hippocastanum were inoculated with bacteria containing the pRiA4 plasmid, with the uid A sequence as a reporter gene. The hairy roots emerging from the wounded sites of androgenic embryos were isolated and maintained in Murashige and Skoog's (MS) liquid hormone-free medium. Five hairy root lines have been maintained in vitro for 4 years with unchanged growth rate and might be a suitable source for secondary metabolite production. The transformation events have been confirmed by a polymerase chain reaction specific to the rol A, B, C and D genes. The absence of residual contaminating bacteria has been shown by a polymerase chain reaction specific to the vir D1 sequence. | en |
dc.description.sponsorship | null | sr |
dc.language.iso | English | sr |
dc.rights | restrictedAccess | |
dc.source | Biologia Plantarum | sr |
dc.title | The horse chestnut lines harboring the rol genes | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Ћалић, Душица Д.; Радојевић, Љиљана Р.; Здравковић-Кораћ, Снежана Р.; Друарт, ПХ; | |
dc.citation.issue | 4 | sr |
dc.citation.volume | 47 | sr |
dc.citation.epage | 491 | sr |
dc.type.version | publishedVersion | en |
dc.citation.rank | M22 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_1777 |
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