Influence of sodium salicylate on rosmarinic acid, carnosol and carnosic acid accumulation by Salvia officinalis L. shoots grown in vitro
2015
Аутори:
Kračun-Kolarević, MargaretaDmitrović, Slavica
Filipović, Biljana
Peric, Marija
Misic, Danijela
Simonović, Ana
Todorović, Slađana
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
To evaluate sodium salicylate (NaSA) as an elicitor of rosmarinic acid
(RA) and phenolic diterpenes, carnosol (C) and carnosic acid (CA)
production, in a culture of Salvia officinalis shoots.
In sage shoots grown in vitro, 28 polyphenolic compounds (phenolic
acids, flavonoids, and phenolic diterpenes) were identified. In shoots
treated for 1 week with increasing NaSA concentrations, the content of C
increased from 2.3 in control to 5.7 mg g(-1) DW in shoots treated with
500 A mu M NaSA. In shoots that were recovered on basal medium for 3
weeks, the maximal amount of C (14 mg/g(-1) DW) was with 150 A mu M NaSA
treatment. In treated and recovered shoots, the increase in C was
accompanied with a decrease in CA, resulting in 1.9-fold increase in the
C/CA ratio. Accumulation of RA was not affected by the NaSA treatment.
However, elicitation by NaSA was accompanied with growth retardation.
NaSA can improve C production in sage shoot culture, probably by
stimulating the conversion of CA to C.
Кључне речи:
Carnosic acid; Carnosol; Elicitors; In vitro; Sage; Salicylic acid; Salvia; Secondary metabolitesИзвор:
Biotechnology Letters, 2015, 37, 8, 1693-1701
DOI: 10.1007/s10529-015-1825-1
ISSN: 1573-6776