Effects of 5-Fluorouracil, Etoposide and CdCl2 in Aquatic Oligochaeta Limnodrilus udekemianus Claparede (Tubificidae) Measured by Comet Assay
2015
Autori:
Kračun-Kolarević, MargaretaKolarević, Stoimir
Atanacković, Ana
Marković, Vanja
Gačić, Zoran
Paunović, Momir
Vuković-Gačić, Branka
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© Springer International Publishing Switzerland 2015
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Genotoxicity of 5-fluorouracil (5-FU), etoposide (ET) and cadmium
chloride (CdCl2) was evaluated in Limnodrilus udekemianus, cosmopolitan
tubificid species, by alkaline single-cell gel electrophoresis (comet
assay). Groups of 50 individuals were exposed in vivo in water-only
short-term (96 h) tests to 5-FU (0.004, 0.04, 0.4, 4 and 40 mu M), ET
(0.004. 0.04, 0.4 and 4 mu M) and CdCl2 (0.004, 0.04, 0.4, 4 and 40 mu
M). Mortality of worms was observed only for CdCl2 (4 and 40 mu M). Cell
viability lower than 70 \% was detected for 5-FU (0.4, 4 and 40 mu M),
ET (4 mu M) and CdCl2 (0.4 and 4 mu M). All tested substances induced
significant increase of DNA damage except 0.004 mu M of ET. L.
udekemianus being sensitive to all tested substances indicates that it
can be used in ecogenotoxicology studies. Concern should be raised to
cytostatics, especially to 5-FU, since concentration of 0.004 mu M
induced DNA damage is similar to ones detected in wastewaters.
Ključne reči:
Limnodrilus udekemianus; 5-Fluorouracil; Etoposide; CdCl2; Genotoxicity; Comet assayFinansiranje / projekti:
- Fate and effects of cytostatic pharmaceuticals in the environment and the identification of biomarkers for and improved risk assessment on environmental exposure (EU-FP7-265264)
- Managing the effects of multiple stressors on aquatic ecosystems under water scarcity (EU-FP7-603629)
- Merenje i modeliranje fizičkih, hemijskih, bioloških i morfodinamičkih parametara reka i vodnih akumulacija (RS-MESTD-Technological Development (TD or TR)-37009)
U:
- Water Air and Soil Pollution (2015), 226(242)
DOI: 10.1007/s11270-015-2511-6
ISSN: 1573-2932
PubMed: 27546921
WoS: 000359369600022
Scopus: 2-s2.0-84958057878
URI
https://radar.ibiss.bg.ac.rs/handle/123456789/1933http://link.springer.com/article/10.1007%2Fs11270-015-2511-6