Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 1: The role of antioxidant enzymes
2015
Аутори:
Filipović, BiljanaSimonović, Ana
Trifunović-Momčilov, Milana
Dmitrović, Slavica
Savic, Jelena M.
Jevremović, Slađana
Subotić, Angelina
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Centaurium erythraea Rafn. is a medicinal plant rich in secoiridoids and
xanthones and used for gastrointestinal disorders, fever, anemia and
many other conditions. C. erythraea is characterized with extraordinary
developmental plasticity and manageability in vitro; thus we propose it
as an excellent experimental model system for studies in developmental
biology. Hereby we describe regeneration of centaury from leaf explants
that can proceed via somatic embryogenesis or organogenesis on inductive
media containing 2,4-D and CPPU. In the absence of growth regulators,
shoots and roots appeared without callusing, on light or in darkness,
respectively. Indirect somatic embryogenesis was induced in the presence
of growth regulators occurring both on light and in darkness. Light was
obligatory for indirect shoot development, where adventitious buds
formed simultaneously with somatic embryos. Dynamic changes of
antioxidative activities of superoxide dismutase (SOD), catalase (CAT)
and peroxidase (POX) in response to morphogenetic changes were followed
during developmental pathways in vitro. Wounding of centaury leaves
immediately induced all SOD and CAT isoforms but caused a decrease in
POX activity. In control leaves and leaf explants, three Cu/Zn-SOD
activities were detected, which gradually decreased on inductive
treatments on light, but remained unchanged during growth in darkness.
Morphogenetic paths on all hormonal and light treatments where
characterized with dynamic changes of CAT activity (comprised of three
major CAT isoforms), but generally CAT was reduced during morphogenesis
induction. POX activity was strongly induced during morphogenesis in all
treatments.
Кључне речи:
Catalase; Morphogenesis; Peroxidase; Superoxide dismutaseИзвор:
Plant Cell Tissue and Organ Culture, 2015, 121, 3, 703-719
DOI: 10.1007/s11240-015-0740-4
ISSN: 1573-5044