Dinamičke asocijacije transkripcionih faktora sa nuklearnim matriksom jetre pacova su funkcionalno povezane sa diferencijalnom genskom ekspresijom alfa-2-makroglobulina
Dynamic associations of transcription factors with the rat liver nuclear matrix are functionally related to differential alpha-2-macroglobulin gene expression
2008
Authors:
Dinić, SvetlanaMihailović, Mirjana
Ivanović Matić, Svetlana
Uskoković, Aleksandra
Grdović, Nevena
Vidaković, Melita
Poznanović, Goran
Document Type:
Article (Published version)
,
© 2008 by the Serbian Biological Society
Metadata
Show full item recordAbstract:
Participation of the nuclear matrix in regulation of alpha-2-macroglobulin (α2M) gene transcription during rat liver development and the acute-phase (AP) response are examined. DNA affinity chromatography of fetal and adult liver internal nuclear matrix proteins under basal and AP conditions with the α2M gene promoter (-852/+12) and immunoblot analysis revealed diverse patterns of association of transcription factors with the nuclear matrix. HNF-6, C/EBPα, and STAT5b were involved in basal and C/EBPβ, STAT1, and STAT3 in AP-stimulated α2M expression. These findings support the assumption that transcription factor-nuclear matrix interactions serve to channel gene regulatory proteins to DNA sequences.
Keywords:
Alpha-2-macroglobulin; Acute phase response; rat liver; nuclear matrix; transcription factorsSource:
Archives of Biological Sciences, 2008, 60, 3, 355-366Funding / projects:
- Akutni i hronični stres: mehanizmi regulacije homeostaze u akutnoj radijacionoj bolesti i dijabetesu (RS-MESTD-MPN2006-2010-143002)
DOI: 10.2298/ABS0803355D
ISSN: 0354-4664