Chemical characterization and biological activity of Chaga (Inonotus obliquus), a medicinal "mushroom"
2015
Аутори:
Glamočlija, JasminaĆirić, Ana
Nikolić, Miloš
Fernandes, Angela
Barros, Lillian
Calhelha, Ricardo C.
Ferreira, Isabel C. F. R.
Soković, Marina
van Griensven, Leo J. L. D.
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Ethnopharmacological relevance: In Russian traditional medicine, an
extract from the mushroom Inonotus obliquus (Fr.) Pil at is used as an
anti-tumor medicine and diuretic. It has been reported that Inonotus
obliquus has therapeutic effects, such as anti-inflammatory,
immuno-modulatory and hepatoprotective effects. This study was designed
to investigate the chemical composition and biological properties of
aqueous and ethanolic extracts of Inonotus obliquus from Finland,
Russia, and Thailand. Their antioxidative, antimicrobial, and antiquorum
properties were tested as well as the cytotoxicity on various tumor cell
lines.
Materials and methods: The tested extract was subjected to conventional
chemical study to identified organic acids and phenolic compounds.
Antioxidative activity was measured by several different assays.
Antimicrobial potential of extracts was tested by microdilution method,
and antiquorum sensing activity and antibiofilm formation of Inonotus
obliquus extracts was tested on Pseudomonas aeruginosa. Cytotoxicity of
the extracts was tested on tumor cells (MCF-7, NCI-H460, HeLa and
HepG(2)) and non-tumor liver cells primary cultures.
Results: Oxalic acid was found as the main organic acid, with the
highest amount in the aqueous extract from Russia. Gallic,
protocatechuic and p-hydroxybenzoic acids were detected in all samples.
Inonotus obliquus extracts showed high antioxidant and antimicrobial
activity. Extracts were tested at subMIC for anti-quorum sensing (AQS)
activity in Pseudomonas aeruginosa and all extracts showed definite AQS
activity. The assays were done using twitching and swarming of bacterial
cultures, and the amount of produced pyocyanin as QS parameters. All the
extracts demonstrated cytotoxic effect on four tumor cell lines and not
on primary porcine liver cells PLP2.
Conclusions: As the Inonotus obliquus presence in Chaga conks is
limited, further purification is necessary to draw quantitative
conclusions. The presence of AQS activity in medicinal mushrooms
suggests a broader anti-infectious disease protection than only
immunomodulatory effects. (C) 2015 Elsevier Ireland Ltd. All rights
reserved.
Кључне речи:
Inonotus obliquus; Chemical characterization; Antioxidant properties; Antimicrobial activity; Antiquorum effect; Antitumor activityИзвор:
Journal of Ethnopharmacology, 2015, 162, 323-332
DOI: 10.1016/j.jep.2014.12.069
ISSN: 0378-8741