In vitro anti-quorum sensing activity of phytol
Abstract:
Anti-quorum sensing activity of the diterpene phytol was evaluated in
vitro for the first time. This compound (at three sub-MIC concentrations
- 0.5, 0.25 and 0.125 MIC, respectively) reduced the formation of
Pseudomonas aeruginosa PAO1 biofilm in the range of 74.00-84.33\%
exhibiting higher activity than the both positive controls used,
streptomycin and ampicillin. Phytol (0.5 MIC) also effectively reduced
P. aeruginosa twitching and flagella motility. Indeed, the bacteria
treated were incapable of producing a twitching zone and had almost
round, smooth and regular colony edges. Finally, the tested compound
(0.5 MIC) exhibited good P. aeruginosa pyocyanin inhibitory activity
(51.94\%) practically to the same extent as streptomycin (52.09\%).
According to the experimental data obtained, this phytol property may
inspire design of medical foods targeting P. aeruginosa quorum sensing
activity.
Keywords:
biofilm; diterpene alcohol; Pseudomonas aeruginosa PAO1; pyocyanin; twitching and flagella motilitySource:
Natural Product Research, 2015, 29, 4, 374-377
DOI: 10.1080/14786419.2014.945088
ISSN: 1478-6427