Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval Weighted Spectra Averaging: Changes in Membrane Properties and Current Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde
2015
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Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
The physiological and biochemical factors that lead to cell death have
not been recognized completely. To our knowledge, there are no data on
the bioelectric parameters that characterize early period of cell death,
as well as on the appearance of related membrane current frequencies. We
studied early parameters of glutaraldehyde (GA)-induced cell death, by
examining the membrane properties of mouse microglia using the
whole-cell patch-clamp technique. In addition, we investigated the
GA-induced changes in the membrane current frequency, to see if
characteristic frequencies would appear in dying cell. For data
analysis, we applied a new approach, an improved multiple moving window
length analysis and interval weighted spectra averaging (IWSA). We chose
GA for its ability to induce almost instantaneous cell death. The 0.6 \%
GA did not induce changes in the bioelectric membrane properties of
microglia. However, the 3 \% GA caused significant decrease of membrane
capacitance and resistance accompanied by the prominent increase in the
membrane currents and nearly ohmic current response of microglial cells.
These data indicate that 3 \% GA caused complete loss of the membrane
function consequently inducing instantaneous cell death. The membrane
function loss was characterized by appearance of the 1.26-4.62 Hz
frequency peak in the IWSA spectra, while no significant increase of
amplitudes could be observed for cells treated with 0.6 \% GA. To our
knowledge, this is the first record of a frequency associated with
complete loss of the membrane function and thus can be considered as an
early indicator of cell death.
Ključne reči:
Microglia; Interval weighted spectra averaging; Membrane current frequency; Patch-clamp; Whole-cell currentIzvor:
Journal of Membrane Biology, 2015, 248, 1, 117-123
DOI: 10.1007/s00232-014-9748-7
ISSN: 0022-2631