BRAIN INJURY ALTERS ECTONUCLEOTIDASE ACTIVITIES AND ADENINE NUCLEOTIDE LEVELS IN RAT SERUM
2015
Authors:
Laketa, DanijelaSavić, Jasmina
Bjelobaba, Ivana
Lavrnja, Irena
Vasić, Vesna
Stojiljković, Mirjana
Nedeljković, Nadežda
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Background: Cortical stab injury (CSI) induces changes in the activity,
expression and cellular distribution of specific ectonucleotidases at
the injury site. Also, several experimentally induced neuropathologies
are associated with changes in soluble ectonucleotidase activities in
the plasma and serum, whilst various insults to the brain alter purine
compounds levels in cerebrospinal fluid, but also in serum, indicating
that insults to the brain may induce alterations in nucleotides release
and rate of their hydrolysis in the vascular system. Since adenine
nucleotides and adenosine regulate diverse cellular functions in the
vascular system, including vascular tone, platelet aggregation and
inflammatory responses of lymphocytes and macrophages, alterations of
ectonucleotidase activities in the vascular system may be relevant for
the clinical outcome of the primary insult.
Methods: We explored ectonucleotidase activities using specific enzyme
assays and determined adenine nucleotides concentrations by the UPLC
method in the rat serum after cortical stab injury.
Results: At 4-h post-injury, ATP and AMP hydrolysis increased by about
60\% and 40\%, respectively, while phosphodiesterase activity remained
unchanged. Also, at 4-h postinjury a marked decrease in ATP
concentration and more than 2-fold increase in AMP concentration were
recorded.
Conclusions: CSI induces rapid up-regulation of nucleotide catabolizing
soluble ectonucleotidases in rat serum, which leads to the observed
shift in serum nucleotide levels. The results obtained imply that
ectonucleotidases and adenine nucleotides participate in the
communication between the brain and the vascular system in physiological
and pathological conditions and thereby may be involved in the
development of various human neuropathologies.
Keywords:
ectonucleotidases; extracellular adenine nucleotides; brain injury; ratSource:
Journal of Medical Biochemistry, 2015, 34, 2, 215-222Funding / projects:
- Cellular and molecular basis of neuroinflamation: potential targets for translational medicine and therapy (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41014)
- Studies of enzyme interactions with toxic and pharmacologically active molecules (RS-MESTD-Basic Research (BR or ON)-172023)
DOI: 10.2478/jomb-2014-0025
ISSN: 1452-8266