Morpho-histological and bioherbicidal evaluation of wild-type and transformed hairy roots of goosefoot
2015
Authors:
Dmitrović, SlavicaBanjac, Nevena
Budimir, Snežana
Janošević, D.
Živković, Suzana
Skorić, Marijana
Ninković, Slavica
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Chenopodium murals L. wild-type roots (WR) and hairy roots (HR) exudates
have been previously shown to exert allelopathic activity against test
plant species lettuce, wheat and Arabidopsis. To further investigate
their allelopathic nature, a comparative morpho-histological
characterization and total phenolic content (TPC) evaluation were
performed. Furthermore, the phytotoxic activity of WR and HR clone R5
against three weed species and their antioxidant responses were also
assessed. Except for the higher degree of branching and root hair
incidence in HR clones, both WR and HR showed similar anatomical
features, typical for higher plants. No significant difference in total
phenolic content between WR and HRs, nor their exudates was found. Root
exudates of WR and R5, applied as growth medium (GM) wherein these C.
murale roots were cultured, displayed selective phytotoxic activity
depending on the target weed species, ranging from suppression of
germination to inhibition of seedling growth, that were linked with
alterations in antioxidant enzyme activities. R5 greatly inhibited
germination and seedling growth of Capsella bursa-pastoris, while WR
inhibited those of Vicia angustifolia and Amaranthus retroflexus. Such
phytotoxic properties (of WR and R5) qualify them as a promising natural
resource in the management of weeds. Differences in allelopathic
activity between HR and WR should be searched for in their
allelochemical profile and the content of each allelopathic substance.
The lack of anatomical abnormalities in hairy roots renders them an
efficient tool for functional-genomic studies of C. murale root genes.
(C) 2014 SAAB. Published by Elsevier B.V. All rights reserved.
Keywords:
Chenopodium murals; Total phenolic content; Bioherbicidal activity; Histological analysis; Vicia angustifolia; Capsella bursa-pastoris; Amaranthus retroflexus; Growth medium; Allelopathy; Antioxidative enzymes; Hairy rootsSource:
South African Journal of Botany, 2015, 96, 53-61Funding / projects:
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
- Biotechnology in vitro - crop, medicinal and endangered plant species (RS-MESTD-Basic Research (BR or ON)-173015)
DOI: 10.1016/j.sajb.2014.11.002
ISSN: 1727-9321