Production of hairy root cultures of lettuce (Lactuca sativa L.)
2014
Аутори:
Tadić, VojinMilošević, Snežana
Cingel, Aleksandar
Petrić, Marija
Trifunović-Momčilov, Milana
Antonić Reljin, Dragana
Jovan, Tadic
Subotić, Angelina
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Hairy root cultures of lettuce (Lactuca sativa L.) were obtained by
inoculation of cotyledonary leaves of in vitro lettuce seedlings (cvs.
Nansen and Ljubljanska ledenka) with Agrobacterium rhizogenes A4M70GUS.
Approximately in 96.7\% cvs. Nansen and in 91.2\% Ljubljanska ledenka
inoculated explants produced hairy root when they were incubated on
Murashige and Skoog (MS) half-strength medium without plant growth
regulators. A total of 54\% of all hairy root cultures expressed GUS
activity. Every hairy root represented an independent transformation
event. Line Ljubljanska ledenka 18 showed the highest biomass (5.5 times
the biomass of control root). A PCR analysis of the genomic DNA
confirmed the presence of marker and target genes in 15 hairy roots
examined.
Кључне речи:
Genetic Transformation; Agrobacterium Rhizogenes; LettuceИзвор:
Central European Journal of Biology, 2014, 9, 12, 1196-1205Финансирање / пројекти:
- Развој и примена биотехнолошких поступака у добијању здравог садног материјала украсних биљака (RS-MESTD-Technological Development (TD or TR)-31019)
DOI: 10.2478/s11535-014-0351-9
ISSN: 1895-104X