Antimicrobial and cytotoxic activities of Alnus rugosa L. aerial parts and identification of the bioactive components
2014
Аутори:
Rashed, KhaledĆirić, Ana
Glamočlija, Jasmina
Calhelha, Ricardo C.
Ferreira, Isabel C. F. R.
Soković, Marina
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
This present investigation was undertaken to study antimicrobial and
cytotoxic activities of dichloromethane, ethyl acetate, and methanol
80\% extracts from Alnus rugosa L. aerial parts, as well investigate the
bio-active compounds of each extract. Antimicrobial activity of the
mentioned extracts against various bacteria and fungi using
microdilution method, and cytotoxicity against five different human
tumor cell lines, and in a non-tumor liver cells primary culture were
tested. Extracts were subjected to phytochemical analysis and column
chromatography eluted with different solvents, n-hexane,
dichloromethane, ethyl acetate, and methanol gradually. The results
showed that the extracts had a significant antimicrobial activity
against all the tested bacteria and fungi, and proved a significant
cytotoxic effect. Methanol 80\% extract exhibited the highest
antibacterial effect, and ethyl acetate extract was the most active as
antifungal agent while dichloromethane extract showed the lowest
antifungal effect. Dichloromethane extract was the most active against
all the tested tumor cell lines. Chromatographic separation of
dichloromethane extract of A. rugosa aerial parts resulted in the
isolation and identification of beta-sitosterol, beta-sitosterol
3-O-beta-glucoside and apigenin, while from ethyl acetate extract,
diosmetin, naringenin, luteolin, and luteolin 7-O-beta-glucoside were
identified, and from methanol 80\% extract, quercetin
3-O-alpha-rhamnoside, isorhamnetin 3-O-beta-glucoside and isorhamnetin
3-O-beta-glucoside 7-O-alpha-rhamnoside were identified. In conclusion,
the present research provided significant information about
antimicrobial and cytotoxic activities and compounds isolated from A.
rugosa aerial parts extracts. (C) 2014 Elsevier B.V. All rights
reserved.
Кључне речи:
Alnus rugosa; Antibacterial activity; Antifungal activity; Anticancer activity; Natural drugs; PhytochemicalsИзвор:
Industrial Crops and Products, 2014, 59, 189-196
DOI: 10.1016/j.indcrop.2014.05.017
ISSN: 1872-633X