Changes in antioxidant enzyme activities in the livers and gills of three cyprinids after exposure to a cyanobacterial bloom in the Gruza Reservoir, Serbia
2014
Authors:
Gavrilović, BrankaDespotović, Svetlana
Gavrić, Jelena
Borković Mitić, Slavica
Ognjanovic, Branka I.
Pavlović, Slađan
Saičić, Zorica
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
We examined the influence of an Aphanizomenon flos-aquae bloom on the
activities of antioxidant enzymes in the livers and gills of three
cyprinids, Rutilus rutilus (roach), Blicca bjoerkna (white bream) and
Carassius gibelio (Prussian carp) from the Gruza Reservoir (Serbia). We
also studied tissue- and species-specific differences in the antioxidant
response to the cyanobacterial bloom. The activities of total, copper
zinc and manganese containing superoxide dismutases (Tot SOD, CuZn SOD,
Mn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione
reductase (GR) and the biotransformation phase II enzyme
glutathione-S-transferase (GST) were determined before and during the
cyanobacterial bloom. B. bjoerkna exhibited higher antioxidant response
to the cyanobacterial bloom than R. rutilus and C gibelio; liver Tot
SOD, CuZn SOD and GST activities increased during the bloom only in this
species. Liver Mn SOD activity in all three cyprinids increased during
the bloom. A decrease in liver CAT and GSH-Px activities that was
detected in all three species may be directly responsible for an
enhanced susceptibility of fish to potential oxidative stress caused by
the bloom. Tot SOD, CuZn SOD, Mn SOD, GSH-Px, GR and GST activities in
gills were also inhibited during the bloom showing that the gills were
affected more than the liver. The observed changes in the activities of
aforementioned antioxidant enzymes during the cyanobacterial bloom
suggest that these parameters could be important biomarkers for
ecotoxicological risk in fish that inhabit ecosystems where A.
flos-aquae blooms occur. (C) 2013 Elsevier Ltd. All rights reserved.
Keywords:
Cyanobacterial bloom; Cyprinid fish; Antioxidant enzymes; Liver; Gills; Aphanizomenon flos-aquaeSource:
Ecological Indicators, 2014, 38, 141-148
DOI: 10.1016/j.ecolind.2013.10.016
ISSN: 1872-7034