Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce
2014
Autori:
Tadić, VojinPetrić, Marija
Milosevic, Snezana
Cingel, Aleksandar
Raspor, Martin
Spasojevic, Dragica
Tadic, Jovan
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
In this study we examined the activities of polyphenol oxidase (PPO) and
antioxidant enzymes, peroxidase (POX) and catalase (CAT) during lettuce
seed germination at different concentrations of phenol. Out of eleven
varieties of lettuce, four were chosen according to their germination
tolerance to phenol as follows: plants exhibiting high (Ljubljanska
ledenka - LJL and Nansen - N) and low toleranace (Little Gem - LG and
Majska kraljica - MK). A decrease in germination efficiency after
exposure to LD50 of phenol was determined for these four varieties. The
effects of phenol treatment on POX, CAT and PPO activities were
determined after 4, 5, 6, 7 and 8 days of growth at LD50 concentrations.
A trend of increased peroxidase activity was observed in seeds grown on
LD50 of phenol compared to control seeds. A significant increase in CAT
activity was observed at the beginning of treatment for MK, LG and N in
seeds grown on phenol as well as in control seeds. A trend of increased
PPO activity was observed in all control seeds. We also investigated the
affinity of PPO for two different substrates that were used for the
determination of enzyme activity. Our results show that LJL and N are
the varieties most tolerant to growth on phenol. Here we report on the
activities of their antioxidant enzymes and PPO during seed germination.
Ključne reči:
Lettuce seeds; phenol; antioxidant enzymes; polyphenol oxidaseIzvor:
Archives of Biological Sciences, 2014, 66, 4, 1503-1514
DOI: 10.2298/ABS1404503T
ISSN: 1821-4339