Regulacija produkcije labdanskih diterpena u kulturi in vitro Cistus creticus L. i analiza njihove biološke aktivnosti
Regulation of labdane diterpenes production in Cistus creticus L. in vitro culture and analysis of their biological activity.
2014
Authors:
Skorić, MarijanaContributors
Todorović, SlađanaSabovljević, Aneta
Glamočlija, Jasmina
Simonović, Ana
Document Type:
Doctoral thesis (Published version)
Metadata
Show full item recordAbstract:
Cistus creticus L. is a perennial, bushy plant that inhabits mainly dry and warm
areas of the Mediterranean. The resin of this species has been actively collected by the
local inhabitants of Crete since ancient times due to its aromatic and medicinal
properties. The predominant bioactive component of the resin and the shoots of this
plant are labdane diterpenes, while the chemical composition of the roots is still
unknown. In order to find the optimal conditions for the labdane diterpenes production,
the subject of this thesis was to investigate the regulation of labdane-type diterpenes
production in in vitro grown plants of C. creticus, as influenced by various abiotic
factors and growth media with varying content of different carbohydrates. In vitro
culture of C. creticus was successfully established on hormone-free MS medium, and
the parameters of growth and development of explants showed no significant
differences among analyzed clones. Optimal concentrations of carbohydrates for in vitro
growth and development of C. creticus ranged from 0.03 M to 0.1 M sucrose or
glucose. Genetic transformation of C. creticus was performed with Agrobacterium
rhizogenes A4M70GUS and the culture of transformed roots was established.
Regeneration of transformed plants was unsuccessful. Histochemical analysis of the
leaves from in vitro cultured C. creticus shoots showed that labdane diterpenes and
phenolic compounds accumulate in the type II capitate glandular trichomes.
Phytochemical characterization of in vitro grown C. creticus has enabled the
identification and quantification of two major groups of secondary metabolites (terpenes
and phenols), which are produced under these conditions. Predominant compounds
accumulating in shoots of C. creticus grown in vitro were labdane diterpenes, the most
abundant among them being labd-7,13-diene-15-ol and labd-7,13-dien-15-ol acetate.
Amongst the volatile secondary metabolites, the most abundant were monoterpenes and
sesquiterpenes. Labd-7,13-dien-15-ol acetate was the most abundant compound in
dichloromethane extracts of type II capitate trichomes exudates. In the roots of C.
creticus, the presence of the phenolic compounds and fatty acids was determined,
whereas labdane diterpenes were not detected...
Keywords:
Cistus creticus L.; Labdane diterpenes; In vitro culture; Phenolic compounds; MVA and MEP biosynthetic pathway; Antioxidant activity; Antimicrobial activity; Cytotoxic activitySource:
University of Belgrade, Faculty of Biology, 2014, 1-207Funding / projects:
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
URI
http://eteze.bg.ac.rs/application/showtheses?thesesId=2922https://fedorabg.bg.ac.rs/fedora/get/o:11142/bdef:Content/download
http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024924338
http://nardus.mpn.gov.rs/123456789/5446
https://radar.ibiss.bg.ac.rs/handle/123456789/2418