Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay
2016
Authors:
Deutschmann, BjörnKolarević, Stoimir
Brack, Werner
Kaisarević, Sonja
Kostić, Jovana
Kračun-Kolarević, Margareta
Liska, Igor
Paunović, Momir
Seiler, Thomas-Benjamin
Shao, Ying
Sipos, Sandor
Slobodnik, Jaroslav
Teodorović, Ivana
Vuković-Gačić, Branka
Hollert, Henner
Document Type:
Article (Published version)
,
© 2016 Elsevier B.V.
Metadata
Show full item recordAbstract:
The Joint Danube Survey 3 (JDS3; the biggest river expedition in 2013) had offered the unique opportunity for a large-scale monitoring approach for biomarker response in feral fish collected along a Danube stretch from Kehlheim (DE) to Sulina (RO). The advantage of genotoxicity as a marker for pollution exposure in fish is the early detection of possible long-term effects such as cancer. Therefore, genotoxicity was in the focus of the biomarker investigations in fish during the expedition. Blood samples of common bleak (Alburnus alburnus) for the investigation of the micronucleus frequency and comet tail intensity of fragmented DNA material in erythrocytes were collected at 18 and 12 sampling sites, respectively. For 9 sampling sites same samples were used to compare the . in-situ data for the comparable genotoxic endpoint in the micronucleus (MN) and comet assay (CM). The data of both . in-situ assays showed a significant correlation, indicating the strength and comparability of the data sets. Significant variation in DNA damage in fish along the longitudinal profile of the Danube was demonstrated for both assays compared to reference sites. The results suggest that DNA damage in erythrocytes of fish was mainly affected by wastewater of highly populated regions. No linkage between the results and the general health/dietary status of the fish were revealed, whereas correlation with some genotoxicity drivers in the water phase, suspended particulate matter and sediments could be demonstrated.
Keywords:
Comet assay; Danube; Fish; Genotoxicity; In-situ; Joint Danube Survey 3; Micronucleus assaySource:
Science of The Total Environment, 2016, 573, 1441-1449Funding / projects:
- Solutions for present and future emerging pollutants in land and water resources management (EU-FP7-603437)
DOI: 10.1016/j.scitotenv.2016.07.175
ISSN: 0048-9697
PubMed: 27519325
WoS: 000390071000139
Scopus: 2-s2.0-84994242298
URI
http://linkinghub.elsevier.com/retrieve/pii/S0048969716316308https://radar.ibiss.bg.ac.rs/handle/123456789/2533