Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative / antioxidative status
2017
Tip dokumenta:
Članak u časopisu (Recenzirana verzija)
,
© TÜBİTAK
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Reduced migration capacity of trophoblast cells leads to poor placentation and correlates with severe pregnancy disorders such as intrauterine growth restriction and preeclampsia. Neuropeptide Y (NPY) is sympathetic cotransmitter involved in various physiological processes and its levels are significantly increased in preeclamptic pregnancy compared to healthy pregnancy. In this study the prooxidative role of NPY and its effects on migration capacity of human trophoblast cell line JEG-3 were investigated together with the effects of nitric oxide (NO) depletion, a molecule that was shown to play an important role in promoting cell migration. The cells were treated for 24 h (short-term stimulation) and 72 h (long-term stimulation) respectively with 1 nM NPY. Oxidative/antioxidative status and the migration index of cells were measured. The results showed increased concentrations of oxidative stress parameters (O2 •–, H2O2) and molecules of the antioxidant defense system (reduced glutathione and oxidized glutathione), while the levels of intracellular nitrites (indicators of NO) and cell migration index were significantly decreased in trophoblast cells treated with NPY (both at 24 h and 72 h of exposure) compared to the control cells. These results suggest that NPY may significantly contribute to reduced migration capacity of trophoblast cells by generating oxidative stress and reducing the bioavailability of NO.
Napomena:
The final publication is available at http://journals.tubitak.gov.tr/biology/issues/biy-17-41-2/biy-41-2-5-1606-73.pdf
Ključne reči:
Neuropeptide Y; Choriocarcinoma trophoblasts; Oxidative stress; Nitric oxide; MigrationIzvor:
Turkish Journal of Biology, 2017, 41, 292-301Finansiranje / projekti:
- Molekularno fiziološki biomonitoring aerobnih organizama zasnovan na određivanju biohemijskih biomarkera oksidacionog stresa (RS-MESTD-Basic Research (BR or ON)-173041)
Povezane informacije:
- Druga verzija
https://radar.ibiss.bg.ac.rs/handle/123456789/2739
DOI: 10.3906/biy-1606-73
ISSN: 1300-0152
WoS: 000400179800005
Scopus: 2-s2.0-85018810634
URI
internal-pdf://Matić et al. - 2016 - Neuropeptide Y reduces migration capacity of human choriocarcinoma cell line by altering oxidative antioxidative.pdfhttp://online.journals.tubitak.gov.tr/openInPressDocument.htm?fileID=799024&no=150073&fileType=Report Document
https://radar.ibiss.bg.ac.rs/handle/123456789/2614