Morphometric analysis of the folliculostellate cells and luteinizing hormone gonadotropic cells of the anterior pituitary of the men during the aging process.
2017
Authors:
Čukuranović Kokoris, JovanaJovanović, Ivan
Pantović, Vukica
Krstić, Miljan
Stanojković, Milica
Milošević, Verica
Ugrenović, Slađana
Stojanović, Vesna
Document Type:
Article (Published version)
,
© 2016 Elsevier Ltd.
Metadata
Show full item recordAbstract:
The aim of this research was to quantify the changes in the morphology and density of the anterior pituitary folliculostellate (FS) and luteinizing hormone (LH) cells. Material was tissue of the pituitary gland of the 14 male cadavers. Tissue slices were immunohistochemically stained with monoclonal anti-LH antibody and polyclonal anti-S100 antibody for the detection of LH and FS cells, respectively. Digital images of the stained slices were afterwards morphometrically analyzed by ImageJ. Results of the morphometric analysis showed significant increase of the FS cells volume density in cases older than 70 years. Volume density of the LH cells did not significantly change, whereas their area significantly increased with age. Nucleocytoplasmic ratio of the LH cells gradually decreased and became significant after the age of 70. Finally, volume density of the FS cell significantly correlated with LH cells area and nucleocytoplasmic ratio. From all above cited, we concluded that in men, density and size of the FS cells increase with age. Long-term hypertrophy of the LH cells results in their functional decline after the age of 70. Strong correlation between FS cells and LH cells morphometric parameters might point to age-related interaction between these two cell groups.
Keywords:
Aging; Folliculostellate cells; Gonadotropes; MorphometrySource:
Tissue & Cell, 2017, 49, 1, 78-85Funding / projects:
- Etiology, diagnostics, prevention and treatment of endemic nephropathy and associated urothelial cancer - importance of genome and proteome study (RS-MESTD-Basic Research (BR or ON)-175092)
DOI: 10.1016/j.tice.2016.11.006
ISSN: 0040-8166
PubMed: 27884532
WoS: 000395508900011
Scopus: 2-s2.0-85007014870
URI
http://linkinghub.elsevier.com/retrieve/pii/S0040816616302464http://www.ncbi.nlm.nih.gov/pubmed/27884532
https://radar.ibiss.bg.ac.rs/handle/123456789/2684