Sex Difference in Oxidative Stress Parameters in Spinal Cord of Rats with Experimental Autoimmune Encephalomyelitis: Relation to Neurological Deficit
2017
Аутори:
Dimitrijević, MirjanaKotur-Stevuljević, Jelena
Stojić-Vukanić, Zorica
Vujnović, Ivana
Pilipović, Ivan
Nacka-Aleksić, Mirjana
Leposavić, Gordana
Тип документа:
Чланак у часопису (Објављена верзија)
,
© Springer Science+Business Media New York 2016
Метаподаци
Приказ свих података о документуАпстракт:
The study examined (a) whether there is sex difference in spinal cord and plasma oxidative stress profiles in Dark Agouti rats immunised for experimental autoimmune encephalomyelitis (EAE), the principal experimental model of multiple sclerosis, and (b) whether there is correlation between the oxidative stress in spinal cord and neurological deficit. Regardless of rat sex, with the disease development xanthine oxidase (XO) activity and inducible nitric oxide synthase (iNOS) mRNA expression increased in spinal cord, whereas glutathione levels decreased. This was accompanied by the rise in spinal cord malondialdehyde level. On the other hand, with EAE development superoxide dismutase (SOD) activity decreased, while O2− concentration increased only in spinal cord of male rats. Consequently, SOD activity was lower, whereas O2− concentration was higher in spinal cord of male rats with clinically manifested EAE. XO activity and iNOS mRNA expression were also elevated in their spinal cord. Consistently, in the effector phase of EAE the concentration of advanced oxidation protein product (AOPP) was higher in spinal cord of male rats, which exhibit more severe neurological deficit than their female counterparts. In as much as data obtained in the experimental models could be translated to humans, the findings may be relevant for designing sex-specific antioxidant therapeutic strategies. Furthermore, the study indicated that the increased pro-oxidant–antioxidant balance in plasma may be an early indicator of EAE development. Moreover, it showed that plasma AOPP level may indicate not only actual activity of the disease, but also serve to predict severity of its course.
Кључне речи:
Experimental autoimmune encephalomyelitis; Multiple sclerosis; Oxidative stress; Rat; Sex differences; Spinal cordФинансирање / пројекти:
- Пластичност имунског система током старења: имуномодулаторни потенцијал естрогена (RS-MESTD-Basic Research (BR or ON)-175050)
У:
- Neurochemical Research (2017), 42(2): 481-492
DOI: 10.1007/s11064-016-2094-7
ISSN: 0364-3190
PubMed: 27812760
WoS: 000395621100015
Scopus: 2-s2.0-84994311207
URI
http://link.springer.com/10.1007/s11064-016-2094-7https://radar.ibiss.bg.ac.rs/handle/123456789/2685