Primena akvatičnih oligoheta (Tubificidae) u in situ i ex situ ekogenotoksikološkim istraživanjima
Application of aquatic oligochaetes (Tubificidae) in ecogenotoxicological in situ and ex situ studies
2017
Authors:
Kračun-Kolarević, MargaretaContributors
Vuković-Gačić, BrankaPaunović, Momir
Gačić, Zoran
Document Type:
Doctoral thesis (Published version)
Metadata
Show full item recordAbstract:
According to the number of individuals and their biomass, family Tubificidae (Oligochaeta) is one of the most important groups in the communities of aquatic macroinvertebrates. As bioindicators these organisms are used in the assessment of the ecological potential of aquatic ecosystems. In this study aquatic oligochaetes are used as experimental organisms in ecogenotoxicological assessment. For in situ studies impact of different stressors on the integrity of the DNA molecule of coelomocytes and haemocytes was evaluated, while the impact of a selected group of pollutants was studied ex situ. The comet assay was used for the evaluation of the genotoxic potential and parameter TI (Tail intensity) for the assessment of the level of DNA damage.In situ research was conducted on the species Branchiura sowerby and in the mixed sampled of tubificid species from the sampling sites Duboko (Sava) and Višnjica (Danube). Coelomocytes and haemocytes have shown to be sensitive to environmental stress. The level of DNA damage in different species showed a similar trend in response to environmental conditions and significantly correlated with microbial, physical and chemical water quality parameters. Water level was recognized as an important stressor that had significant impact on the other environmental parameters. Different stressors (floods, wastewater discharge, and runoffs) did not affect the number of individuals and the diversity of aquatic oligochaetes populations. On the other hand, the impact of stressors was evident on the level of DNA damage.The effects of cytostatic, 5-fluorouracil and etoposide and of model mutagen CdCl2, on the level of DNA damage of Limnodrilus udekemianus was examined ex situ. All tested substances significantly increased the level of DNA damage.
Keywords:
In situ and ex situ studies; Microbial and chemical pollution; Cytostatics; Aquatic Oligochaeta; Family Tubificidae; Ecogenotoxicology; Comet assay; DNA damageFunding / projects:
- Monitoring and Modeling of Rivers and Reservoirs (MORE) - Physical, Chemical, Biological and Morphodynamic Parameters (RS-MESTD-Technological Development (TD or TR)-37009)
- Fate and effects of cytostatic pharmaceuticals in the environment and the identification of biomarkers for and improved risk assessment on environmental exposure (EU-FP7-265264)
- Managing the effects of multiple stressors on aquatic ecosystems under water scarcity (EU-FP7-603629)
- Projekat Austrijskog fonda za nauku (Austrian Science Fund –FWF) – „Establishing the basic microbial faecal pollution pattern along a large river: testing the longitudinal continuum vs. lateral discontinuum hypothesis at the River Danube“
In:
- University of Belgrade, Faculty of Biology (2017)
URI
http://eteze.bg.ac.rs/application/showtheses?thesesId=5016https://fedorabg.bg.ac.rs/fedora/get/o:15686/bdef:Content/download
http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=48978447
http://nardus.mpn.gov.rs/123456789/8268
https://radar.ibiss.bg.ac.rs/handle/123456789/2799