Plant regeneration from in vitro-derived leaf and petiole explants of Viola cornuta L. 'Lutea Splendens'
2017
Аутори:
Antonić Reljin, DraganaTrajković, Milena
Cingel, Aleksandar
Subotić, Angelina
Jevremović, Slađana
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2009, Sejani Publisher
Метаподаци
Приказ свих података о документуАпстракт:
In vitro-derived petiole and leaf explants of Viola cornuta L. 'Lutea Splendens' were cultured on half-strength Murashige and Skoog (MS) medium with several concentrations of 6-benzylaminopurin (BAP), 6-furfurylaminopurine (KIN), N-phenyl-N'(1,2,3-thidiazol-5-yl) urea (TDZ) or N-(2-chloro-4-pyridyl)-N'phenylurea (CPPU) alone or supplemented with indole-3-acetic acid (IAA) or a-naphthaleneacetic acid (NAA). The most efficient direct adventitious shoot induction (21%) without callus formation was obtained using petiole explants cultured on half-strength MS medium containing 2.02 μM CPPU. Significantly higher shoot multiplication was achieved on medium supplemented with TDZ (7.6 shoots) or CPPU (7.1 shoots) than on medium supplemented with NAA and BAP (2.9 shoots). Shoots were rooted on half-strength MS medium without plant growth regulators.
Кључне речи:
CPPU; Horned pansy; Regeneration; TDZИзвор:
Propagation of Ornamental Plants, 2017, 17, 3, 95-102Финансирање / пројекти:
- Развој и примена биотехнолошких поступака у добијању здравог садног материјала украсних биљака (RS-MESTD-Technological Development (TD or TR)-31019)
URI
http://www.journal-pop.org/2017_17_3_95-102.htmlhttps://radar.ibiss.bg.ac.rs/handle/123456789/2905