Plant regeneration from in vitro-derived leaf and petiole explants of Viola cornuta L. 'Lutea Splendens'
2017
Autori:
Antonić Reljin, DraganaTrajković, Milena
Cingel, Aleksandar
Subotić, Angelina
Jevremović, Slađana
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© 2009, Sejani Publisher
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
In vitro-derived petiole and leaf explants of Viola cornuta L. 'Lutea Splendens' were cultured on half-strength Murashige and Skoog (MS) medium with several concentrations of 6-benzylaminopurin (BAP), 6-furfurylaminopurine (KIN), N-phenyl-N'(1,2,3-thidiazol-5-yl) urea (TDZ) or N-(2-chloro-4-pyridyl)-N'phenylurea (CPPU) alone or supplemented with indole-3-acetic acid (IAA) or a-naphthaleneacetic acid (NAA). The most efficient direct adventitious shoot induction (21%) without callus formation was obtained using petiole explants cultured on half-strength MS medium containing 2.02 μM CPPU. Significantly higher shoot multiplication was achieved on medium supplemented with TDZ (7.6 shoots) or CPPU (7.1 shoots) than on medium supplemented with NAA and BAP (2.9 shoots). Shoots were rooted on half-strength MS medium without plant growth regulators.
Ključne reči:
CPPU; Horned pansy; Regeneration; TDZIzvor:
Propagation of Ornamental Plants, 2017, 17, 3, 95-102Finansiranje / projekti:
- Razvoj i primena biotehnoloških postupaka u dobijanju zdravog sadnog materijala ukrasnih biljaka (RS-MESTD-Technological Development (TD or TR)-31019)
URI
http://www.journal-pop.org/2017_17_3_95-102.htmlhttps://radar.ibiss.bg.ac.rs/handle/123456789/2905