Fructose-enriched diet induces inflammation and reduces antioxidative defense in visceral adipose tissue of young female rats.
2017
Тип документа:
Чланак у часопису (Објављена верзија)
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© 2015, Springer-Verlag Berlin Heidelberg.
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PURPOSE The consumption of refined, fructose-enriched food continuously increases and has been linked to development of obesity, especially in young population. Low-grade inflammation and increased oxidative stress have been implicated in the pathogenesis of obesity-related disorders including type 2 diabetes. In this study, we examined alterations in inflammation and antioxidative defense system in the visceral adipose tissue (VAT) of fructose-fed young female rats, and related them to changes in adiposity and insulin sensitivity. METHODS We examined the effects of 9-week fructose-enriched diet applied immediately after weaning on nuclear factor κB (NF-κB) intracellular distribution, and on the expression of pro-inflammatory cytokines (IL-1β and TNFα) and key antioxidative enzymes in the VAT of female rats. Insulin signaling in the VAT was evaluated at the level of insulin receptor substrate-1 (IRS-1) protein and its inhibitory phosphorylation on Ser307. RESULTS Fructose-fed rats had increased VAT mass along with increased NF-κB nuclear accumulation and elevated IL-1β, but not TNFα expression. The protein levels of antioxidative defense enzymes, mitochondrial manganese superoxide dismutase 2, and glutathione peroxidase, were reduced, while the protein content of IRS-1 and its inhibitory phosphorylation were not altered by fructose diet. CONCLUSIONS The results suggest that fructose overconsumption-related alterations in pro-inflammatory markers and antioxidative capacity in the VAT of young female rats can be implicated in the development of adiposity, but do not affect inhibitory phosphorylation of IRS-1.
Кључне речи:
Female rats; Fructose diet; Inflammation; NF-κB; Oxidative stress; Visceral adipose tissueИзвор:
European journal of nutrition, 2017, 56, 1, 151-160Финансирање / пројекти:
- Улога стероидних хормона у неуроендокриној адаптацији на стрес и патофизиологији метаболичког синдрома - молекуларни механизми и клиничке импликације (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41009)
DOI: 10.1007/s00394-015-1065-0
ISSN: 1436-6207
PubMed: 26433940
WoS: 000394168100013
Scopus: 2-s2.0-84944566005
URI
http://link.springer.com/10.1007/s00394-015-1065-0http://www.ncbi.nlm.nih.gov/pubmed/26433940
https://radar.ibiss.bg.ac.rs/handle/123456789/2917