Research on Chemical Composition and Biological Properties Including Antiquorum Sensing Activity of Angelica pancicii Vandas Aerial Parts and Roots
2017
Autori:
Mileski, Ksenija S.Trifunović, Snežana S.
Ćirić, Ana
Šakić, Željana M.
Ristić, Mihailo S.
Todorović, Nina M.
Matevski, Vlado S.
Marin, Petar D.
Tešević, Vele V.
Džamić, Ana M.
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© 2017 American Chemical Society.
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
The essential oil, different extracts, and isolated compounds of Angelica pancicii Vandas (Apiaceae) were investigated for the first time. The GC-FID and GC-MS analyses revealed sesquiterpenoids as the main constituents of A. pancicii essential oil of aerial parts with bornyl acetate (8.08%), n-octanol (5.82%), kessane (4.26%), and β-selinene (4.26%) as the main constituents. Analysis of methanol extracts, using an HPLC-DAD/ESI-ToF-MS system, showed a total of 52 compounds in the aerial parts and 53 in the roots, indicating coumarins as the main constituents. In addition, new chromone (1) and six known furanocoumarins (2-7) were isolated from the roots and structurally elucidated by combined spectroscopic methods. The aerial part extracts exhibited higher polyphenolic contents and antioxidant activity evaluated by three radical scavenging assays. Using a microwell dilution method, the strongest antibacterial activity profiles were determined for ethanol and methanol root extracts (minimum bactericidal concentrations (MBCs) = 0.25-3.00 mg/mL), which were comparable to the activity of streptomycin (MBCs = 0.34-1.24 mg/mL), while the strongest antibacterial compound of A. pancicii was oxypeucedanin hydrate (MBCs = 0.50-8.00 mg/mL). Antifungal potential was in moderate extent, and the highest activity was obtained for root methanol extract (minimum fungicidal concentrations (MFCs) = 4.00-14.00 mg/mL). Tested sub-minimum inhibitory concentrations (subMICs) of the extracts and isolated compounds inhibited selected Pseudomonas aeruginosa PAO1 virulence determinants. The most reduced growth of P. aeruginosa colony was in the presence of isolated oxypeucedanin. Ethanol (17.36-46.98%) and methanol (34.54-52.43%) root extracts showed higher anti-biofilm activity compared to streptomycin (49.40-88.36%) and ampicillin (56.46-92.16%).
Ključne reči:
Angelica pancicii; Antimicrobial; Antioxidant; Antiquorum sensing activity; Chemical composition; Coumarins; Essential oilIzvor:
Journal of Agricultural and Food Chemistry, 2017, 65, 50, 10933-10949Finansiranje / projekti:
- Mikromorfološka, fitohemijska i molekularna istraživanja biljaka - sistematski, ekološki i primenljivi aspekti (RS-MESTD-Basic Research (BR or ON)-173029)
- Bioaktivni prirodni proizvodi samoniklih, gajenih i jestivih biljaka: određivanje struktura i aktivnosti (RS-MESTD-Basic Research (BR or ON)-172053)
DOI: 10.1021/acs.jafc.7b04202
ISSN: 0021-8561
PubMed: 29129053
WoS: 000418783400013
Scopus: 2-s2.0-85038626982
URI
http://pubs.acs.org/doi/10.1021/acs.jafc.7b04202https://radar.ibiss.bg.ac.rs/handle/123456789/2952