Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models.
2018
Autori:
Kolarević, StoimirMilovanović, Dragana
Kračun-Kolarević, Margareta
Kostić, Jovana
Sunjog, Karolina
Martinović, Rajko
Đorđević, Jelena
Novaković, Irena
Sladić, Dušan
Vuković-Gačić, Branka
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
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© 2017 Informa UK Limited, trading as Taylor & Francis Group
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
In this study, mutagenic and genotoxic potential of anti-tumor compounds avarol, avarone, and its derivatives 3'-methoxyavarone, 4'-(methylamino)avarone and 3'-(methylamino)avarone was evaluated and compared to cytostatics commonly used in chemotherapy (5-fluorouracil, etoposid, and cisplatin). Mutagenic potential of selected hydroquinone and quinones was assessed in prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002. Genotoxic potential was also assessed in eukaryotic models using comet assay in human fetal lung cell line (MRC-5), human adenocarcinoma epithelial cell line (A549), and in human peripheral blood cells (HPBC). The results indicated that avarol and avarone do not exert mutagenic/genotoxic potential. Among the studied avarone derivatives, mutagenic potential was detected by SOS/umuC test for 3'-(methylamino)avarone, but only after metabolic activation. The results of comet assay indicated that 3'-methoxyavarone and 3'-(methylamino)avarone have a significant impact on the level of DNA damage in the MRC-5 cell line. Genotoxic potential was not observed in A549 cells or HPBC probably due to a different uptake rate for the compounds and lower in metabolism rate within these cells.
Ključne reči:
Avarol; SOS/umuC; Avarone; Comet assay; Genotoxicity; MutagenicityIzvor:
Drug and Chemical Toxicology, 2018, 42, 2, 130-139Finansiranje / projekti:
- Biološki aktivni prirodni proizvodi kao potencijalni izvori novih lekova i dijetetskih suplemenata (RS-MESTD-Basic Research (BR or ON)-172058)
- Interakcije prirodnih proizvoda, njihovih derivata i kompleksnih jedinjenja sa proteinima i nukleinskim kiselinama (RS-MESTD-Basic Research (BR or ON)-172055)
DOI: 10.1080/01480545.2017.1413108
ISSN: 0148-0545
PubMed: 29299944
WoS: 000459818600003
Scopus: 2-s2.0-85041102861
URI
https://www.tandfonline.com/doi/full/10.1080/01480545.2017.1413108https://radar.ibiss.bg.ac.rs/handle/123456789/2966