Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing.
2018
Authors:
Zmejkoski, DanicaSpasojević, Dragica
Orlovska, Irina
Kozyrovska, Natalia
Soković, Marina
Glamočlija, Jasmina
Dmitrović, Svetlana
Matović, Branko
Tasić, Nikola
Maksimović, Vuk
Sosnin, Mikhail
Radotić, Ksenija
Document Type:
Article (Published version)
,
© 2018 Elsevier B.V.
Metadata
Show full item recordAbstract:
Lignins and lignin-derived compounds are known to have antibacterial properties. The wound healing agents in the form of dressings produce faster skin repair and decrease pain in patients. In order to create an efficient antimicrobial agent in the form of dressing in the treatment of chronic wounds, a composite hydrogel of bacterial cellulose (BC) and dehydrogenative polymer of coniferyl alcohol (DHP), BC-DHP, was designed. Novel composite showed inhibitory or bactericidal effects against selected pathogenic bacteria, including clinically isolated ones. The highest release rate of DHP was in the first hour, while after 24 h there was still slow release of small amounts of DHP from BC-DHP during 72 h monitoring. High-performance liquid chromatography coupled with mass-spectrometry showed that BC-DHP releases DHP oligomers, which are proposed to be antimicrobially active DHP fractions. Scanning electron microscopy and atomic force microscopy micrographs proved a dose-dependent interaction of DHP with BC, which resulted in a decrease of the pore number and size in the cellulose membrane. The Fourier-transform infrared absorption spectra of the BC-DHP showed that DHP was partly bound to the BC matrix. The swelling and crystallinity degree were dose-dependent. All obtained results confirmed BC-DHP composite as a promising hydrogel for wounds healing.
Keywords:
Antimicrobial activity; Bacterial cellulose; Lignin model polymerSource:
International Journal of Biological Macromolecules, 2018, 118, Pt A, 494-503Funding / projects:
- Synthesis, processing and characterization of nanostructured materials for application in the field of energy, mechanical engineering, environmental protection and biomedicine (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45012)
- Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering (RS-MESTD-Basic Research (BR or ON)-173017)
- Zero- to Three-Dimensional Nanostructures for Application in Electronics and Renewable Energy Sources: Synthesis, Characterization and Processing (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45007)
- The membranes as sites of interaction between the intracellular and apoplastic environments: studies of the bioenergetics and signaling using biophysical and biochemical techniques. (RS-MESTD-Basic Research (BR or ON)-173040)
- Characterization and application of fungal metabolites and assessment of new biofungicides potential (RS-MESTD-Basic Research (BR or ON)-173032)
- National Academy of Sciences of Ukraine (grant 47/2015-2016)
DOI: 10.1016/j.ijbiomac.2018.06.067
PubMed: 29909035
WoS: 000445170200057
Scopus: 2-s2.0-85049108073
URI
https://www.sciencedirect.com/science/article/pii/S0141813018320919?via%3Dihubhttps://radar.ibiss.bg.ac.rs/handle/123456789/3101