Daidzein upregulates anti-aging protein Klotho and NaPi 2a cotransporter in a rat model of the andropause.
2019
Authors:
Živanović, JasminaJarić, Ivana
Ajdžanović, Vladimir
Mojić, Marija
Miler, Marko
Šošić-Jurjević, Branka
Milošević, Verica
Filipović, Branko
Document Type:
Article (Published version)
,
© 2018 Elsevier GmbH
Metadata
Show full item recordAbstract:
In a rat model of the andropause we aimed to examine the influence of daidzein, soy isoflavone, on the structure and function of parathyroid glands (PTG) and the expression levels of some of the crucial regulators of Ca2+ and Pi homeostasis in the kidney, and to compare these effects with the effects of estradiol, serving as a positive control. Middle-aged (16-month-old) male Wistar rats were divided into the following groups: sham-operated (SO), orchidectomized (Orx), orchidectomized and estradiol-treated (Orx+E; 0.625mg/kg b.w./day, s.c.) as well as orchidectomized and daidzein-treated (Orx+D; 30mg/kg b.w./day, s.c.) group. Every treated group had a corresponding control group. PTH serum concentration was decreased in Orx+E and Orx+D groups by 10% and 21% (p<0.05) respectively, in comparison with the Orx. PTG volume was decreased in Orx+E group by 16% (p<0.05), when compared to the Orx. In Orx+E group expression of NaPi 2a was lower (p<0.05), while NaPi 2a abundance in Orx+D animals was increased (p<0.05), when compared to Orx. Expression of PTH1R was increased (p<0.05) in Orx+E group, while in Orx+D animals the same parameter was decreased (p<0.05), in comparison with Orx. Klotho expression was elevated (p<0.05) in Orx+D rats, in regard to Orx. Orx+D induced reduction in Ca2+/creatinine and Pi/creatinine ratio in urine by 32% and 16% (p<0.05) respectively, in comparison with Orx. In conclusion, presented results indicate the more coherent beneficial effects of daidzein compared to estradiol, on disturbed Ca2+ and Pi homeostasis, and presumably on bone health, in the aging male rats.
Keywords:
Andropause; Daidzein; Klotho; Parathyroid gland; Rat; Sodium phosphate cotransporter 2aSource:
Annals of Anatomy = Anatomischer Anzeiger, 2019, 221, 27-37Funding / projects:
- The effects of select plant extracts, phytoestrogens, steroid and peptide hormones on the rat neuroendocrine system (RS-MESTD-Basic Research (BR or ON)-173009)
- Molecular mechanisms of physiological and pharmacological control of inflammation and cancer (RS-MESTD-Basic Research (BR or ON)-173013)
DOI: 10.1016/j.aanat.2018.08.001
ISSN: 0940-9602
PubMed: 30240906
WoS: 000458710200003
Scopus: 2-s2.0-85053773528
URI
https://www.sciencedirect.com/science/article/pii/S0940960218301080?via%3Dihubhttps://radar.ibiss.bg.ac.rs/handle/123456789/3150