Antioxidative effects of phenolic extracts from chestnut leaves, catkins and spiny burs in streptozotocin-treated rat pancreatic β-cells
2011
Authors:
Mujić, AidaGrdović, Nevena
Mujić, Ibrahim
Mihailović, Mirjana
Živković, Jelena
Poznanović, Goran
Vidaković, Melita
Document Type:
Article (Published version)
,
© 2010 Elsevier Ltd.
Metadata
Show full item recordAbstract:
Oxidative stress plays a central role in the onset of diabetes which is characterised by progressive pancreatic β-cell failure. We studied the ability of phenolic extracts prepared from chestnut leaf, catkin and spiny burs to prevent oxidative stress-induced β-cell (Rin-5F) death. The Rin-5F cell death was induced by treatment with the diabetogenic agent streptozotocin (STZ) and the protective effects of the chestnut extracts were assessed after cells treatment with a STZ/extracts mixture. The chestnut extracts increased cell viability by protecting DNA from oxidative damage and by enhancing the natural antioxidant system. Moreover, the chestnut extracts had a significant impact on the cell's redox-status which was observed as an increase in the levels of reduced glutathione. Chestnut extracts were capable of inhibiting lipid peroxidation, which was manifested as a lowering of malondialdehyde levels. We concluded that chestnut extracts increased cell viability after STZ treatment as a result of their antioxidative properties. © 2010 Elsevier Ltd.
Keywords:
Antioxidative activity; Cell death; DNA damage; Oxidative stress; Phenolic-rich chestnut extractsSource:
Food Chemistry, 2011, 125, 3, 841-849Funding / projects:
- Akutni i hronični stres: mehanizmi regulacije homeostaze u akutnoj radijacionoj bolesti i dijabetesu (RS-MESTD-MPN2006-2010-143002)
DOI: 10.1016/j.foodchem.2010.08.068
ISSN: 0308-8146