Chemical and Morphological Inter- and Intrapopulation Variability in Natural Populations of Gentiana pneumonanthe L.
2019
Authors:
Popović, ZoricaKrstić Milošević, Dijana
Marković, Milena
Matić, Rada
Vidaković, Vera
Bojović, Srđan
Document Type:
Article (Published version)
,
© 2019 Wiley-VHCA AG, Zurich, Switzerland
Metadata
Show full item recordAbstract:
Inter- and intrapopulation variability in six natural populations of the rare species Gentiana pneumonanthe was examined based on morphological and chemical data. Population size and linear morphometric parameters differed significantly among populations, but without a clear connection to habitat conditions, i. e. water supply and light availability. Leaf shape varied from ovate to lanceolate in all populations, and one population was distinctive in having the largest number of leaves of transitional shape. HPLC analyses of six secondary metabolites were performed separately for belowground parts, and aboveground vegetative and reproductive parts of individual plants (6 populations ×7 individuals ×3 plant parts, n=126) in order to examine differences at the population and individual levels. Three secoiridoids (swertiamarin (SWM), sweroside (SWZ), and gentiopicrin (GP)), one xanthone (mangiferin (MGF)), and two flavones (isoorientin (IO) and isovitexin (IV)) were detected and quantified in the analyzed samples: sweroside dominated in the aboveground reproductive part, mangiferin in the aboveground vegetative part, and gentiopicrin in the belowground part. At the population level, differences in contents of the analyzed chemicals among populations were significant only for a few metabolites. At the individual level, a pronounced organ-dependent distribution of secondary metabolites was revealed. The results of this study contribute to a better understanding of natural variability within populations of the rare and threatened G. pneumonanthe, and provide data on the contents and within-plant distribution of secondary metabolites, which are important as pharmacologically active compounds and may be useful for further biotechnological procedures regarding this species.
Keywords:
Gentiana pneumonanthe; Biological activity; Mangiferin; Marsh gentian; Natural habitat; Natural products; Population variability; SecoiridoidsSource:
Chemistry & Biodiversity, 2019, 16, 2, e1800509-Funding / projects:
- Evaluation of ecophysiological and genetic plant diversity in forest ecosystems (RS-MESTD-Basic Research (BR or ON)-173011)
- Biotechnology in vitro - crop, medicinal and endangered plant species (RS-MESTD-Basic Research (BR or ON)-173015)
- Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46008)
DOI: 10.1002/cbdv.201800509
PubMed: 30511810
WoS: 000458565500020
Scopus: 2-s2.0-85061093576
URI
https://onlinelibrary.wiley.com/doi/abs/10.1002/cbdv.201800509https://radar.ibiss.bg.ac.rs/handle/123456789/3260