Protective effects of whey on rat liver damage induced by chronic alcohol intake.
2019
Authors:
Radić, IMijović, M
Tatalović, Nikola
Mitić, M
Lukić, V
Joksimović, B
Petrović, Z
Ristić, S
Veličković, S
Nestorovic, V
Corac, A
Mirić, M
Adžić, M
Blagojević, Duško
Popović, L
Hudomal, S J
Document Type:
Article (Accepted Version)
,
© The Author(s) 2019.
Metadata
Show full item recordAbstract:
In 2012, alcohol liver disease resulted in 3.3 million-5.9% of global deaths. This study introduced whey protection capacity against chronic alcohol-induced liver injury. Rats were orally administered to 12% ethanol solution in water (ad libitum, average 8.14 g of ethanol/kg body weight (b.w.)/day) alone or combined with whey ( per os, 2 g/kg b.w./day). After 6-week treatment, chronic ethanol consumption induced significant histopathological liver changes: congestion, central vein dilation, hepatic portal vein branch dilation, Kupffer cells hyperplasia, fatty liver changes, and hepatocytes focal necrosis. Ethanol significantly increased liver catalase activity and glutathione reductase protein expression without significant effects on antioxidative enzymes: glutathione peroxidase (GPx), copper-zinc-containing superoxide dismutase (CuZnSOD) and manganese-containing superoxide dismutase (MnSOD). Co-treatment with whey significantly attenuated pathohistological changes induced by ethanol ingestion and increased GSH-Px and nuclear factor kappa B (NF-κB) protein expression. Our results showed positive effects of whey on liver chronically exposed to ethanol, which seem to be associated with NF-κB-GPx signaling.
Keywords:
Alcohol liver disease; NF-κB; Antioxidant enzymes; Ethanol; Rat; WheySource:
Human & Experimental Toxicology, 2019Funding / projects:
- Molecular mechanisms of redox signalling in homeostasis: adaptation and pathology (RS-MESTD-Basic Research (BR or ON)-173014)
- Defining a cluster of molecular biomarkers for improved diagnostics and therapy of mood disorders (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41029)
DOI: 10.1177/0960327119829518
PubMed: 30784321
WoS: 000470714400002
Scopus: 2-s2.0-85062018247
URI
http://journals.sagepub.com/doi/10.1177/0960327119829518https://radar.ibiss.bg.ac.rs/handle/123456789/3281