Evaluation of the antioxidative enzymes in the seminal plasma of infertile men: Contribution to classic semen quality analysis.
2019
Authors:
Otašević, VesnaKalezić, Anđelika
Macanović, Biljana
Janković, Aleksandra
Stančić, Ana
Garalejić, Eliana
Korać, Aleksandra
Korać, Bato
Document Type:
Article (Accepted Version)
,
© 2019 Taylor & Francis
Metadata
Show full item recordAbstract:
Protein expression/activity of antioxidative defense enzymes (AD) in seminal plasma of fertile men might be used as biomarkers of male fertility status. To test this concept, the present study examined the semen parameters of males among 14 normal idiopathic (normozoospermia) and 84 subnormal (teratozoospermia, oligoteratozoospermia, oligoasthenoteratozoospermia) infertile individuals\. We investigated levels of protein expression/activity of Cu, Zn superoxide dismutase (CuZnSOD), manganese superoxide dismutase (MnSOD), catalase and glutathione peroxidase (GSH-Px), their association with functional sperm parameters, as well as their potential to serve as biomarkers of specific sperm pathologies. Although the activity of CuZnSOD and protein expression of catalase were significantly correlated with several sperm parameters, underlying their potential role in etiology of various sperm abnormalities, investigation of their potential usefulness as a biomarker of semen quality showed that these AD enzymes could not distinguish subtle differences between various sperm pathologies. In contrast, GSH-Px activity was decreased in all groups with sperm pathologies and was a very good indicator of aberrations in functional sperm parameters, explaining up to 94.6% of infertility cases where functional sperm parameters were affected. Therefore, assessment of GSH-Px activity showed the potential to discriminate between infertile males with normal and subnormal semen characteristics and may prove useful in the evaluation of male (in)fertility. Abbreviations: AD: antioxidative defense; Cu, Zn SOD: copper, zinc superoxide dismutase; GSH-Px: glutathione peroxidase; MnSOD: manganese superoxide dismutase; NS: normospermia; OATS: oligoasthenoteratozoospermia; OTS: oligoteratozoospermia; ROC: receiver operating characteristic; ROS: reactive oxygen species; TS: teratozoospermia; WHO: world health organization.
Keywords:
Sperm parameters; Antioxidative defense; Biomarker; Male infertility; Seminal plasmaSource:
Systems Biology in Reproductive Medicine, 2019Funding / projects:
- Reactive oxygen and nitrogen species functions in reproduction: possible pharmacological tools to treat human infertility (RS-MESTD-Basic Research (BR or ON)-173054)
DOI: 10.1080/19396368.2019.1600171
PubMed: 30964348
WoS: 000465838700001
Scopus: 2-s2.0-85064090284
URI
https://www.tandfonline.com/doi/full/10.1080/19396368.2019.1600171https://radar.ibiss.bg.ac.rs/handle/123456789/3330