Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.
2019
Autori:
Dmitrović, SlavicaDragićević, Milan
Savić, Jelena
Milutinović, Milica
Živković, Suzana
Maksimović, Vuk
Matekalo, Dragana
Mišić, Danijela
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© 2019 Elsevier GmbH
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Active ingredient of the commercial herbicide BASTA (B), phosphinothricin, acts as an inhibitor of glutamine synthetase (GS), a key enzyme in ammonium assimilation. The treatment with BASTA leads to an elevation of ammonium levels in plants and further to various physiological alterations, ammonium toxicity and lethality. Results of the present study emphasize the complexity underlying control mechanisms that determine BASTA interaction with essential oil (EO) from Nepeta rtanjensis (NrEO), bioherbicide inducing oxidative stress in target plants. Simultaneous application of NrEO and BASTA, two agents showing differential mode of action, suspends BASTA-induced ammonium toxicity in Arabidopsis thaliana plants. This is achieved through maintaining GS activity, which sustains a sub-toxic and/or sub-lethal ammonium concentration in tissues. As revealed by the present study, regulation of GS activity, as influenced by BASTA and NrEO, occurs at transcriptional, posttranscriptional, and/or posttranslational levels. Two genes encoding cytosolic GS, GLN1;1 and GLN1;3, are highlighted as the main isozymes in Arabidopsis shoots contributing to NrEO-induced overcoming of BASTA-generated ammonium toxicity. The effects of NrEO might be ascribed to its major component nepetalactone, but the contribution of minor EO components should not be neglected. Although of fundamental significance, the results of the present study suggest possible low efficiency of BASTA in plantations of medicinal/aromatic plants such as Nepeta species. Furthermore, these results highlight the possibility of using NrEO as a bioherbicide in BASTA-treated crop fields to mitigate the effect of BASTA residues in contaminated soils.
Ključne reči:
Ammonium toxicity; Arabidopsis; Essential oil; Glutamine synthetase; Nepeta; PhosphinothricinIzvor:
Journal of Plant Physiology, 2019, 237, 87-94Finansiranje / projekti:
- Fiziološka, hemijska i molekularna analiza diverziteta odabranih retkih i ugroženih biljnih vrsta u cilju ex situ zaštite i produkcije biološki aktivnih jedinjenja (RS-MESTD-Basic Research (BR or ON)-173024)
- Primene niskotemperaturnih plazmi u biomedicini, zaštiti čovekove okoline i nanotehnologijama (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41011)
- Biotehnologija in vitro - gajene, lekovite i ugrožene biljne vrste (RS-MESTD-Basic Research (BR or ON)-173015)
- Interakcije membrana sa unutarćelijskim i apoplastičnim prostorom: izučavanja bioenergetike i singalizacije koristeći biofizičke i biohemijske metode (RS-MESTD-Basic Research (BR or ON)-173040)
DOI: 10.1016/j.jplph.2019.04.006
PubMed: 31034969
WoS: 000469206100010
Scopus: 2-s2.0-85064662121
URI
https://www.sciencedirect.com/science/article/pii/S0176161719300525?via%3Dihubhttps://radar.ibiss.bg.ac.rs/handle/123456789/3342