Comparative study of oxidative stress parameters and acetylcholinesterase activity in the liver of Pelophylax esculentus complex frogs
2017
Authors:
Prokić, MarkoBorković Mitić, Slavica
Krizmanić, Imre
Gavrić, Jelena
Despotović, Svetlana
Gavrilović, Branka
Radovanović, Tijana
Pavlović, Slađan
Saičić, Zorica
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Comparative activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), the phase II biotransformation enzyme glutathione-S-transferase (GST), the concentrations of total glutathione (GSH), sulfhydryl groups (-SH) and the activity of the neurotoxicity biomarker acetylcholinesterase (AChE) were investigated in the livers of species belonging to the Pelophylax esculentus "complex" (parental species Pelophylax ridibundus, Pelophylax lessonae, and their hybrid Pelophylax kl. esculentus) from the wetland, Obedska bara in Serbia. The condition factor (CF) and hepato somatic index (HSI) were also calculated. All three species were caught at same locality and were exposed to the same environmental conditions. Liver SOD activity was lower in P. ridibundus than in P. kl. esculentus and P. lessonae; higher activities of CAT, GR and GST were observed in P. kl. esculentus frogs as compared to their parental species. The activity of GSH-Px was significantly lower in P. kl. esculentus. The activity of AChE was increased in P. lessonae as compared to P. kl. esculentus and P. ridibundus. Similar concentrations of GSH and -SH groups were observed in all investigated species. P. kl. esculentus had a higher CF, while the HSI was lower when compared to the parental species. Our findings suggest that the parental species (P. ridibundus and P. lessonae) possess more similar antioxidative responses to environmental conditions than the hybrid species P. kl. esculentus. The obtained results improve our understanding of the biology and physiology of these three closely related species.
Keywords:
Acetylcholinesterase; Antioxidative defense system; Frogs; Liver; Oxidative stress; Pelophylax esculentus complexSource:
Saudi Journal of Biological Sciences, 2017, 24, 1, 51-58Funding / projects:
- Molecular and physiological biomonitoring of aerobic organisms based on the determination of biochemical biomarkers of oxidative stress (RS-MESTD-Basic Research (BR or ON)-173041)
DOI: 10.1016/j.sjbs.2015.09.003
PubMed: 28053571
WoS: 000391837000008
Scopus: 2-s2.0-84941249250
URI
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC5198921https://radar.ibiss.bg.ac.rs/handle/123456789/3417