Pyrimethanil: Between efficient fungicide against Aspergillus rot on cherry tomato and cytotoxic agent on human cell lines
2019
Autori:
Aleksić, MilenaStanisavljević, Danijela
Ivanov, Marija
Vasiljević, Perica
Stevanović, Milena
Soković, Marina
Stojković, Dejan
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© 2019 Association of Applied Biologists
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Pyrimethanil, a synthetic fungicide widely used for the treatment of pre‐ and postharvest fungal diseases on different agricultural crops, was explored for its antifungal activity on different fungal strains. The effect of pyrimethanil on fungal ergosterol was tested by using Aspergillus niger as a model organism. Furthermore, it was investigated, if pyrimethanil can effectively reduce the appearance of Aspergillus rot in wounded cherry tomato fruits. The fungicide cytotoxic effect on different human cell lines was evaluated. In addition, its influence on cell proliferation was studied. A. niger was the most resistant fungal strain (MFC 1.88 μg μL−1) to the effect of pyrimethanil. Addition of ergosterol doubled the MFC on A. niger, indicating that the compound might interfere with ergosterol, main sterol of fungal cell membrane. Disease incidence of A. niger in wounded cherry tomato fruits was not detected with pyrimethanil treatment of 0.75 mg/wound. Some cytotoxic effects of pyrimethanil on human cell lines were recorded already at 50 ng μL−1, while the expression of Ki67 marker of proliferation was decreased with 150 ng μL−1. These results altogether indicate that pyrimethanil is effective in reducing various fungal pathogens, but further use of this fungicide should be reevaluated because of its cytotoxicity.
Ključne reči:
Antifungal; Aspergillus rot; Cell proliferation; Cytotoxicity; Pyrimethanil; TomatoIzvor:
Annals of Applied Biology, 2019, 175, 2, 228-235Finansiranje / projekti:
- Karakterizacija i primena metabolita gljiva i utvrđivanje potencijala novih biofungicida (RS-MESTD-Basic Research (BR or ON)-173032)
- Proučavanje signalnih puteva i epigenetičkih mehanizama uključenih u kontrolu ekspresije humanih SOX gena: dalje rasvetljavanje njihove uloge u određivanju sudbine i diferencijaciji ćelija (RS-MESTD-Basic Research (BR or ON)-173051)
URI
https://onlinelibrary.wiley.com/doi/abs/10.1111/aab.12532https://radar.ibiss.bg.ac.rs/handle/123456789/3444