Subchronic Oral Cadmium Exposure Exerts both Stimulatory and Suppressive Effects on Pulmonary Inflammation/Immune Reactivity in Rats.
2019
Autori:
Kulaš, JelenaNinkov, Marina
Tucović, Dina
Popov Aleksandrov, Aleksandra
Ukropina, Mirela
Cakić Milošević, Maja
Mutić, Jelena
Kataranovski, Milena
Mirkov, Ivana
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© 2019 Biomedical and Environmental Sciences
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
OBJECTIVE The aim of this study is to investigate the effects of oral cadmium (Cd) ingestion on the pulmonary immune response. METHODS Determination of Cd content in lungs and histopathological evaluation of the tissue was performed in rats following 30-day oral Cd administration (5 and 50 mg/L). Antioxidant enzyme defense (superoxide dismutase and catalase), cell infiltration, and production of tumor necrosis factor (TNF) and interferon (IFN)-γ, as well as the activity of myeloperoxidase (MPO), nitric oxide (NO), and various cytokines [interleukin (IL)-1β, IL-6, IL-10, and IL-17] were investigated. RESULTS Cd caused tissue damage and cell infiltration in the lungs, and this damage was more pronounced at higher doses. Cd deposition resulted in lung inflammation characterized by a dose-dependent IL-1β increase in lung homogenates, increased TNF levels at both doses, and IL-6 stimulation at low doses with inhibition observed at higher doses. Cd exerted differential effects on lung leukocytes isolated by enzyme digestion, and these effects were characterized by a lack of change in the production of reactive oxygen and nitrogen species, an inhibition of IL-1β and TNF, and stimulation of MPO and IFN-γ. The higher capacity of Cd-exposed lung cells to respond to the opportunistic pathogen Staphylococcus epidermidis was demonstrated in vitro. CONCLUSION The potential of ingested Cd to exert both proinflammatory and immunosuppressive effects on pulmonary tissue inflammation and immune reactivity highlights the complex immunomodulatory actions of this metal.
Ključne reči:
Cytokine response; Lungs; Oral cadmium administration; RatsIzvor:
Biomedical and Environmental Sciences, 2019, 32, 7, 508-519Finansiranje / projekti:
- Imunomodulatorni efekti ksenobiotika i biotičkih faktora životne sredine na populacije mišolikih glodara (RS-MESTD-Basic Research (BR or ON)-173039)
DOI: 10.3967/bes2019.068
PubMed: 31331435
WoS: 000476473000004
Scopus: 2-s2.0-85071830951
URI
http://www.besjournal.com/en/article/doi/10.3967/bes2019.068https://radar.ibiss.bg.ac.rs/handle/123456789/3470