Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration
2019
Authors:
Živanović, JasminaKouroussis, Emilia
Kohl, Joshua B.
Adhikari, Bikash
Bursać, Biljana
Schott-Roux, Sonia
Petrović, Dunja
Miljković, Jan Lj.
Thomas-Lopez, Daniel
Jung, Youngeun
Miler, Marko
Mitchell, Sarah
Milošević, Verica
Gomes, Jose Eduardo
Benhar, Moran
Gonzales-Zorn, Bruno
Ivanović-Burmazović, Ivana
Torregrossa, Roberta
Mitchell, James R.
Whiteman, Matthew
Schwarz, Guenter
Snyder, Solomon H.
Paul, Bindu D.
Carroll, Kate S.
Filipović, Miloš R.
Document Type:
Article (Published version)
,
Crown Copyright © 2019 Published by Elsevier Inc.
Metadata
Show full item recordAbstract:
Life on Earth emerged in a hydrogen sulfide (H2S)-rich environment eons ago and with it protein persulfidation mediated by H2S evolved as a signaling mechanism. Protein persulfidation (S-sulfhydration) is a post-translational modification of reactive cysteine residues, which modulate protein structure and/or function. Persulfides are difficult to label and study due to their reactivity and similarity with cysteine. Here, we report a facile strategy for chemoselective persulfide bioconjugation using dimedone-based probes, to achieve highly selective, rapid, and robust persulfide labeling in biological samples with broad utility. Using this method, we show persulfidation is an evolutionarily conserved modification and waves of persulfidation are employed by cells to resolve sulfenylation and prevent irreversible cysteine overoxidation preserving protein function. We report an age-associated decline in persulfidation that is conserved across evolutionary boundaries. Accordingly, dietary or pharmacological interventions to increase persulfidation associate with increased longevity and improved capacity to cope with stress stimuli.
Keywords:
Hydrogen sulfide; Protein persulfidation; Hydrogen peroxide; Sulfenylation; Sulfinylation; Sulfonylation; Redox signaling; Aging; Calorie restrictionSource:
Cell Metabolism, 2019, 30, 6, 1152-1170.e13Funding / projects:
- The effects of select plant extracts, phytoestrogens, steroid and peptide hormones on the rat neuroendocrine system (RS-MESTD-Basic Research (BR or ON)-173009)
DOI: 10.1016/J.CMET.2019.10.007
ISSN: 1550-4131
PubMed: 31735592
WoS: 000500800300015
Scopus: 2-s2.0-85075485979
URI
https://www.sciencedirect.com/science/article/abs/pii/S1550413119305625https://radar.ibiss.bg.ac.rs/handle/123456789/3518