Silencing of germacrene A synthase genes reduces guaianolide oxalate content in Cichorium intybus L.
2020
Authors:
Bogdanović, MilicaCankar, Katarina
Dragićević, Milan
Bouwmeester, Harro
Beekwilder, Jules
Simonović, Ana
Todorović, Slađana
Document Type:
Article (Published version)
,
© 2019 Taylor & Francis Group, LLC.
Metadata
Show full item recordAbstract:
Chicory (Cichorium intybus L.) is a medicinal and industrial plant from the Asteraceae family that produces a variety of sesquiterpene lactones (STLs), most importantly bitter guaianolides: lactucin, lactucopicrin and 8-deoxylactucin as well as their modified forms such as oxalates. These compounds have medicinal properties; however, they also hamper the extraction of inulin - a very important food industry product from chicory roots. The first step in guaianolide biosynthesis is catalyzed by germacrene A synthase (GAS) which in chicory exists in two isoforms - GAS long (encoded by CiGASlo) and GAS short (encoded by CiGASsh). AmiRNA silencing was used to obtain plants with reduced GAS gene expression and level of downstream metabolites, guaianolide-15-oxalates, as the major STLs in chicory. This approach could be beneficial for engineering new chicory varieties with varying STL content, and especially varieties with reduced bitter compounds more suitable for inulin production.
Keywords:
Cichorium intybus; amiRNA; Gene silencing; Germacrene A synthase; Guaianolides; Sesquiterpene lactonesSource:
GM Crops & Food, 2020, 11, 1, 54-66Funding / projects:
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
- Plant Terpenoids for Human Health: a chemical and genomic approach to identify and produce bioactive compounds (EU-FP7-227448)
DOI: 10.1080/21645698.2019.1681868
ISSN: 2164-5698
PubMed: 31668117
WoS: 000493421600001
Scopus: 2-s2.0-85074904136
URI
https://www.tandfonline.com/doi/full/10.1080/21645698.2019.1681868https://radar.ibiss.bg.ac.rs/handle/123456789/3520