Protective effects of Alchemilla vulgaris L. extracts against cisplatin-induced toxicological alterations in rats
2020
Authors:
Jurić, TatjanaKatanić Stanković, Jelena S.
Rosić, Gvozden
Selaković, Dragica
Joksimović, Jovana
Mišić, Danijela
Stanković, Vesna
Mihailović, Vladimir
Document Type:
Article (Published version)
,
© 2019 SAAB
Metadata
Show full item recordAbstract:
This study was designed to evaluate the possible effect of methanol extracts of aerial parts and roots of Alchemilla vulgaris L. (AVA and AVR, respectively) in preventing cisplatin-induced hepatorenal and testicular toxicity in rats. UHPLC/DAD/(−)HESI-MS/MS analysis was performed to determine the detailed phenolic profile of AVA and AVR. The male Wistar rats were orally treated with extracts at three different concentrations (50, 100, and 200 mg/kg b.w.) for 10 days and toxicity was induced by injection of single dose of cisplatin on the 5th day (7.5 mg/kg b.w.). Determination of serum biochemical markers of hepatorenal and testicular tissue injury, as well as oxidative-stress parameters in tissues and histopathological study, were performed. Treatments with AVA and AVR significantly attenuated the levels of serum parameters of liver, kidneys and testicles injury, tissue's morphology and parameters of oxidative stress caused by an application of the cisplatin. Chromatographic analysis showed the presence of more than 20 different phenolic compounds in extracts where ellagic acid, catechin, and catechin gallate were dominant components in both extracts. The obtained results indicate that A. vulgaris extracts may be used in preventing cisplatin-induced toxicity during chemotherapy as well as in the treatment of oxidative stress-related disorders.
Keywords:
Alchemilla vulgaris L.; Cisplatin; Ellagic acid; Oxidative stressSource:
South African Journal of Botany, 2020, 128, 141-151Funding / projects:
- Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43004)
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
URI
https://www.sciencedirect.com/science/article/pii/S0254629919309962?via%3Dihubhttps://radar.ibiss.bg.ac.rs/handle/123456789/3522