"Does my Diet Affect my Perfume?" Identification and Quantification of Cuticular Compounds in Five Drosophila melanogaster Strains Maintained over 300 Generations on Different Diets
2016
Authors:
Pavković-Lučić, SofijaTodosijević, Marina
Savić, Tatjana
Vajs, Vlatka
Trajković, Jelena
Anđelković, Boban
Lučić, Luka
Krstić, Gordana
Makarov, Slobodan
Tomić, Vladimir
Miličić, Dragana
Vujisić, Ljubodrag
Document Type:
Article (Published version)
,
© Verlag Helvetica Chimica Acta AG, Zürich
Metadata
Show full item recordAbstract:
Cuticular hydrocarbons (CHCs) in Drosophila melanogaster represent the basis of chemical communication being involved in
many important biological functions. The aim of this study was to characterize chemical composition and variation of cuticular
profiles in five D. melanogaster strains. These strains were reared for approximately 300 generations on five diets: standard
cornmeal medium and substrates prepared with apple, banana, tomato, and carrot. Differences in quantity and/or quality in
CHCs were assumed as a result of activation of different metabolic pathways involved in food digestion and adaptations to the
particular diet type.
In total, independently of sex and strain, 66 chemical compounds were identified. In females of all strains, 60 compounds were
identified, while, in males, 47 compounds were extracted. Certain new chemical compounds for D. melanogaster were found.
MANOVA confirmed that CHC amounts significantly depend on sex and substrates, as well as on their interactions. Discriminant
analysis revealed that flies belonging to ÐappleÏ and ÐcarrotÏ strains exhibited the most noticeable differences in CHC repertoires.
A non-hydrocarbon pheromone, cis-vaccenyl acetate (cVA) also contributed to the variation in the pheromone bouquet among
the strains. Variability detected in CHCs and cVAmay be used in the explanation of differences in mating behaviour previously
determined in analyzed fly strains.
Keywords:
Drosophila melanogaster; Cuticular hydrocarbons; Diet; cis‐Vaccenyl acetateSource:
Chemistry & Biodiversity, 2016, 13, 2, 224-232Funding / projects:
- Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-MESTD-Basic Research (BR or ON)-172053)
DOI: 10.1002/cbdv.201500064
ISSN: 1612-1880
PubMed: 26880435